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      Successive digestion of tilapia collagen by serine proteinase and proline specific endopeptidase to produce novel angiotensin I-converting enzyme inhibitory peptides

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          Abstract

          Serine proteinase, purified from the hepatopancreas of Pacific white shrimp ( Litopenaeus vannamei), was used to hydrolyze acid solubilized collagen (ASC) isolated from Nile tilapia ( Oreochromis sp.) skin to produce angiotensin I-converting enzyme (ACE) inhibitory peptides (ACEIPs). A series of column chromatography assays were used to separate the ACEIPs. A peptide, NPARTCR, was isolated as it exhibited high ACE inhibition potential. Further digestion of this peptide by a proline specific endopeptidase (PSEP), produced a pentapeptide ARTCR with ACE inhibitory activity (IC 50) of 77.0 µmol/L. Both NPARTCR and ARTCR inhibited ACE in a non-competitive manner. An in vivo study in rats demonstrated that ARTCR has ACE inhibitory activity via lowering systolic blood pressure in spontaneously hypertensive rats (SHRs). These results suggest that processing by-products from shrimp and tilapia are ideal raw materials for the production of serine proteinase and collagen, respectively. Serine proteinase and collagen are both ideal raw materials that can be used to derive ACE inhibitory active peptides against hypertension.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 August 2020
          14 April 2020
          : 2
          : 3
          : 268-278
          Affiliations
          1College of Food and Biological Engineering, Jimei University, Xiamen 361021, China
          2Collaborative Innovation Center of Marine Food Deep Processing, Dalian Polytechnic University, Dalian 116034, China
          3Department of Biological and Chemical Engineering, Shaoyang University, Shaoyang 422000, China
          4Faculty of Fisheries, Nagasaki University, Nagasaki 852-8521, Japan
          Author notes
          *Corresponding author: Minjie Cao, E-mail: mjcao@ 123456jmu.edu.cn
          Article
          s42995-020-00038-y
          10.1007/s42995-020-00038-y
          © 2020 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Research Paper

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