7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Effectiveness of bone marrow transplantation for revitalizing a severely necrotic small bone: experimental rabbit model

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Although treating Kienböck disease is controversial, we previously applied a new method that was less invasive and comprised drilling, bone marrow (BM) transplantation, external fixation, and radiating low-intensity pulsed ultrasound. We reported good clinical results obtained by this new method, which were comparable to those obtained using other, rather invasive methods. Here, we investigated the effect of drilling holes and transplanting BM into necrotic bone in an animal model to further understand the effect of these methods on the revitalization of necrotic bone. We used rabbit fourth tarsal bones, whose surfaces consist of cartilage and cortical bone, mimicking human lunate bone. We soaked the retrieved bones in liquid nitrogen to induce necrosis. After thawing, we inserted them separately into bilateral subcutaneous pouches in the backs of rabbits. A total of 60 rabbits were divided into four groups of 15 rabbits each: BM transplantation (BM group); peripheral blood transplantation (PB group); drilling (D group); control (C group). We sacrificed three rabbits to obtain six specimens in each group at 2, 4, 8, 12, and 20 weeks after operation and evaluated the specimens histomorphologically. In the BM group, significantly larger mineralizing surfaces, osteoblast surfaces, and osteoclast numbers were observed at 4, 8, and 12 weeks compared with those in the other groups. No significant differences were observed at 2 and 20 weeks in the groups except the mineralizing surface of the 20-week-BM group, which was significantly greater. We examined the efficacy of drilling and of BM transplantation for regenerating necrotic bone in a rabbit model. Our experiments suggest that drilling with BM transplantation to the necrotic bone accelerates bone formation and remodeling.

          Related collections

          Author and article information

          Journal
          Journal of Orthopaedic Science
          Journal of Orthopaedic Science
          Springer Nature
          09492658
          May 2010
          May 2010
          : 15
          : 3
          : 381-388
          Article
          10.1007/s00776-010-1459-z
          20559807
          b04f0215-facc-4698-b8b0-49dc83f0bb89
          © 2010

          https://www.elsevier.com/tdm/userlicense/1.0/

          History

          Comments

          Comment on this article