A novel bocavirus in canine liver

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.

Findings

In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.

Conclusions

We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal’s complex disease remains to be determined.

Related collections

Most cited references 19

Record: found
Abstract: found
Article: not found

Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces.

Liguo Liu, Jiang Du, Lilian Sun … (2012)

Bats are natural hosts for a large variety of zoonotic viruses. This study aimed to describe the range of bat viromes, including viruses from mammals, insects, fungi, plants, and phages, in 11 insectivorous bat species (216 bats in total) common in six provinces of China. To analyze viromes, we used sequence-independent PCR amplification and next-generation sequencing technology (Solexa Genome Analyzer II; Illumina). The viromes were identified by sequence similarity comparisons to known viruses. The mammalian viruses included those of the Adenoviridae, Herpesviridae, Papillomaviridae, Retroviridae, Circoviridae, Rhabdoviridae, Astroviridae, Flaviridae, Coronaviridae, Picornaviridae, and Parvovirinae; insect viruses included those of the Baculoviridae, Iflaviridae, Dicistroviridae, Tetraviridae, and Densovirinae; fungal viruses included those of the Chrysoviridae, Hypoviridae, Partitiviridae, and Totiviridae; and phages included those of the Caudovirales, Inoviridae, and Microviridae and unclassified phages. In addition to the viruses and phages associated with the insects, plants, and bacterial flora related to the diet and habitation of bats, we identified the complete or partial genome sequences of 13 novel mammalian viruses. These included herpesviruses, papillomaviruses, a circovirus, a bocavirus, picornaviruses, a pestivirus, and a foamy virus. Pairwise alignments and phylogenetic analyses indicated that these novel viruses showed little genetic similarity with previously reported viruses. This study also revealed a high prevalence and diversity of bat astroviruses and coronaviruses in some provinces. These findings have expanded our understanding of the viromes of bats in China and hinted at the presence of a large variety of unknown mammalian viruses in many common bat species of mainland China.

0 comments Cited 152 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis.

Joseph Victoria, Amit Kapoor, Linlin Li … (2009)

We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with nonpolio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of capsid-protected, nuclease-resistant viral nucleic acids were followed by DNA sequencing and sequence similarity searches. Limited Sanger sequencing (35 to 240 subclones per sample) identified an average of 1.4 distinct eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses, including rotavirus, adenovirus, picobirnavirus, and human enterovirus species A (HEV-A) to HEV-C, as well as numerous other members of the Picornaviridae family, including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The viruses with the most divergent sequences relative to those of previously reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, and Circoviridae families and the Bocavirus genus). Samples from six healthy contacts of AFP patients were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalences and pathogenicities of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies with different demographic and patient groups, now facilitated by knowledge of these viral genomes.

0 comments Cited 146 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Human bocavirus-the first 5 years.

Klaus Hedman, Olli Ruuskanen, David M. Soderlund … (2011)

Four species of human bocavirus (HBoV) have been recently discovered and classified in the Bocavirus genus (family Parvoviridae, subfamily Parvovirinae). Although detected both in respiratory and stool samples worldwide, HBoV1 is predominantly a respiratory pathogen, whereas HBoV2, HBoV3, and HBoV4 have been found mainly in stool. A variety of signs and symptoms have been described in patients with HBoV infection including rhinitis, pharyngitis, cough, dyspnea, wheezing, pneumonia, acute otitis media, fever, nausea, vomiting, and diarrhea. Many of these potential manifestations have not been systematically explored, and they have been questioned because of high HBoV co-infection rates in symptomatic subjects and high HBoV detection rates in asymptomatic subjects. However, evidence is mounting to show that HBoV1 is an important cause of lower respiratory tract illness. The best currently available diagnostic approaches are quantitative PCR and serology. This concise review summarizes the current clinical knowledge on HBoV species. Copyright © 2011 John Wiley & Sons, Ltd.

0 comments Cited 101 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Linlin Li

Patricia A Pesavento

Christian M Leutenegger

Marko Estrada

Lark L Coffey

Samia N Naccache

Erik Samayoa

Charles Chiu

Jianming Qiu

Chunlin Wang

Xutao Deng

Eric Delwart

Journal

Journal ID (nlm-ta): Virol J

Journal ID (iso-abbrev): Virol. J

Title: Virology Journal

Publisher: BioMed Central

ISSN (Electronic): 1743-422X

Publication date Collection: 2013

Publication date (Electronic): 13 February 2013

Volume: 10

Page: 54

Affiliations

[1 ]Blood Systems Research Institute, San Francisco, CA, USA

[2 ]Department of Laboratory Medicine, University of California, San Francisco, CA, USA

[3 ]Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA

[4 ]IDEXX Reference Laboratories, CA, USA

[5 ]UCSF-Abbott Viral Diagnostics and Discovery Center, CA, USA

[6 ]Department of Microbiology, University of Kansas, KS, USA

[7 ]Stanford Genome Technology Center, Stanford, CA, USA

Article

Publisher ID: 1743-422X-10-54

DOI: 10.1186/1743-422X-10-54

PMC ID: 3577433

PubMed ID: 23402347

SO-VID: b07027c0-585b-49ae-81d3-bf7a04f1231f

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 19 December 2012

Date accepted : 31 January 2013

Product

Self URI: http://www.virologyj.com/content/10/1/54

Comments

Comment on this article

scite_

Cited by 33

See all cited by

Most referenced authors 328

See all reference authors

A novel bocavirus in canine liver

Read this article at

Abstract

Background

Findings

Conclusions

Related collections

Coronaviruses, COVID-19 and SARS-CoV-2

Most cited references 19

Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces.

Metagenomic analyses of viruses in stool samples from children with acute flaccid paralysis.

Human bocavirus-the first 5 years.

Author and article information

Contributors

Journal

Affiliations

Article

History

Product

Categories

Comments

Comment on this article

Similar content 660

Cited by 33

Most referenced authors 328