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      Loss of Immunoreactivity of Human Serum Parathyroid Hormone by the Action of Rat Kidney Enzyme, Preferentially Hydrolyzing Parathyroid Hormone

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          Abstract

          Acetone powder of rat kidney was extracted with0.15 m KCl containing 5 mM tris-Cl and 15% glycerol. The fraction, precipitated by 35-75% saturated ammonium sulfate, was chromatographed on a Biogel P-300 column to obtain a partially purified rat kidney enzyme preparation preferentially hydrolyzing parathyroid hormone. Parathyroid hormone in human serum lost its immunoreactivity after incubation with this rat kidney enzyme preparation according to radioimmunoassay using guinea pig antibody to bovine parathyroid hormone and <sup>125</sup>I-labeled highly purified bovine parathyroid hormone. Such activity of destroying PTH immunoreactivity, proportional to the amount of enzyme and duration of incubation, was lost after heating at 60°C for 10 min. The optimum pH, 8.6, and susceptibility to inhibitors such as p-chloromercuribenzoate, monoidoacetate, o-phenanthroline and EDTA were similar to those in the hydrolysis of <sup>125</sup>I-PTH.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1973
          1973
          21 November 2008
          : 4
          : 4
          : 213-218
          Affiliations
          Department of Geriatrics, University of Tokyo Faculty of Medicine, Tokio
          Article
          178310 Horm Res 1973;4:213–218
          10.1159/000178310
          4201258
          b075d353-f918-45fd-94bb-900560c05d89
          © 1973 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Page count
          Pages: 6
          Categories
          Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Parathyroid hormone,Radioimmunoassay,Immunoreactivity

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