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      Temporal evolution of the Mediterranean fin whale song

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          Abstract

          We present an analysis of fin whale ( Balaenoptera physalus) songs on passive acoustic recordings from the Pelagos Sanctuary (Western Mediterranean Basin). The recordings were gathered between 2008 and 2018 using 2 different hydrophone stations. We show how 20 Hz fin whale pulses can be automatically detected using a low complexity convolutional neural network (CNN) despite data variability (different recording devices exposed to diverse noises). The pulses were further classified into the two categories described in past studies and inter pulse intervals (IPI) were measured. The results confirm previous observations on the local relationship between pulse type and IPI with substantially more data. Furthermore we show inter-annual shifts in IPI and an intra-annual trend in pulse center frequency. This study provides new elements of comparison for the understanding of long term fin whale song trends worldwide.

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          Management of validation of HPLC method for determination of acetylsalicylic acid impurities in a new pharmaceutical product

          The work mainly focused on a validation of the method for determining the content of salicylic acid and individual unknown impurities in new pharmaceutical product—tablets containing: 75, 100 or 150 mg of acetylsalicylic acid and glycine in the amount of 40 mg for each dosage. The separation of the components was carried out by means of HPLC, using a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) as the stationary phase. The mobile phase consisted of a mixture of 85% orthophosphoric acid, acetonitrile and purified water (2:400:600 V/V/V). Detection was carried out at a wavelength of 237 nm, with a constant flow rate of 1.0 ml min −1 . In order to verify the method, linearity, precision (repeatability and reproducibility), accuracy, specificity, range, robustness, system precision, stability of the test and standard solution, limit of quantification and forced degradation were determined. Validation tests were performed in accordance with ICH (International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use) guidelines. The method was validated successfully. It was confirmed that the method in a tested range of 0.005–0.40% salicylic acid with respect to acetylsalicylic acid content is linear, precise and accurate.
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            Orientation by means of long range acoustic signaling in baleen whales.

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              The 20-Hz signals of finback whales (Balaenoptera physalus).

              The 20-Hz signals of finback whales (Balaenoptera physalus) were analyzed from more than 25 years of recordings at a variety of geographic locations on near-surface hydrophones close to whales and on deep hydrophone systems. These signals were composed of 1-s pulses of sinusoidal waveform with downward sweeping frequency from approximately 23 to 18 Hz at variable source levels up to 186 dB (re: 1 microPa at 1 m), usually with slightly lower levels for the pulses at the beginning and end of sequences. These "20-Hz" pulses were produced in signal bouts (separated by more than 2 h) lasting as long as 32.5 h. Bouts were composed of regularly repeated pulses at intervals of 7-26 s (typically), either at one nominal pulse rate or at two alternating (doublet) pulse intervals. Signal bouts were interrupted by rests of 1-20 min at roughly 15-min intervals and by irregular gaps lasting between 20 and 120 min. The distribution of these signals throughout the year and their temporal sequence were analyzed from the continuous drum records of the Bermuda SOFAR Station. Signal bouts occurred during winter, sometimes beginning in September and ending in May. The sound sequences were never exactly replicated. Direct association of the bouts with the reproductive season for this species points to the 20-Hz signals as possible reproductive displays by finback whales.
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                Author and article information

                Contributors
                paul.best@univ-tln.fr
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                9 August 2022
                9 August 2022
                2022
                : 12
                : 13565
                Affiliations
                [1 ]GRID grid.462878.7, ISNI 0000 0000 9766 3011, Université de Toulon, , Aix Marseille Univ, CNRS, LIS, DYNI, ; Marseille, France
                [2 ]Pôle INPS, Marseille , France
                Article
                15379
                10.1038/s41598-022-15379-0
                9363496
                35945237
                b07f02f7-dfc6-4530-8238-3b8b3bab1034
                © The Author(s) 2022

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 17 November 2021
                : 23 June 2022
                Funding
                Funded by: Interreg,European Union
                Award ID: Maritimo GIAS
                Award Recipient :
                Categories
                Article
                Custom metadata
                © The Author(s) 2022

                Uncategorized
                marine biology,acoustics,computer science
                Uncategorized
                marine biology, acoustics, computer science

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