26
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Counting absolute numbers of molecules using unique molecular identifiers.

      Nature Methods

      Animals, Chromatin Immunoprecipitation, methods, Down Syndrome, genetics, Drosophila melanogaster, Female, Gene Library, Genomics, Humans, Karyotyping, Male, RNA, Messenger, analysis, Sequence Analysis, RNA

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Counting individual RNA or DNA molecules is difficult because they are hard to copy quantitatively for detection. To overcome this limitation, we applied unique molecular identifiers (UMIs), which make each molecule in a population distinct, to genome-scale human karyotyping and mRNA sequencing in Drosophila melanogaster. Use of this method can improve accuracy of almost any next-generation sequencing method, including chromatin immunoprecipitation-sequencing, genome assembly, diagnostics and manufacturing-process control and monitoring.

          Related collections

          Author and article information

          Journal
          22101854
          10.1038/nmeth.1778

          Comments

          Comment on this article