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      Serial Alterations of Glomerular Matrix-Degrading Metalloproteinase Activity in Anti-Thymocyte-Induced Glomerulonephritis in Rats

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          Abstract

          In the present study, we investigated the serial changes of glomerular metalloproteinase (GMP) activity in antithymocyte-induced glomerulonephritis (Thy.1 GN) in rats. GMP activity was determined by the measurement of EDTA-inhibitable gelatinolytic activity toward tritiated gelatin as substrate. Thy.1 GN was made by injection of antithymocyte serum into female Wistar rats and glomeruli were separated by a graded sieving method. Glomeruli were homogenized with sonication, and then the supernatant was used for the assay of GMP activity and substrate study. GMP activity was reduced on day 1 and at week 2 compared with controls, and returned to the control level by week 9. On light-microscopic examination, diffuse and focal glomerular ECM expansion were observed at weeks 2 and 4, respectively. These changes disappeared by week 9. With the use of several substrates labeled with tritium, GMP was observed to degrade type IV collagen, fibronectin and casein as well as gelatin, but not type I collagen. In conclusion, attenuated GMP activity may be one of the causes of glomerular ECM expansion in Thy.1 GN, because GMP was capable of degrading the components of glomerular ECM.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1998
          February 1998
          26 January 1998
          : 78
          : 2
          : 195-200
          Affiliations
          a First Department of Internal Medicine, Miyazaki Medical College, Miyazaki, b Third Department of Internal Medicine, Faculty of Medicine, University of Akita, Japan
          Article
          44910 Nephron 1998;78:195–200
          10.1159/000044910
          9496737
          © 1998 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Figures: 4, References: 23, Pages: 6
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          Self URI (application/pdf): https://www.karger.com/Article/Pdf/44910
          Categories
          Original Paper

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