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      Development of genotyping by sequencing (GBS)- and array-derived SNP markers for stem rust resistance gene Sr42

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          A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat.

          Agricultural crops benefit from resistance to pathogens that endures over years and generations of both pest and crop. Durable disease resistance, which may be partial or complete, can be controlled by several genes. Some of the most devastating fungal pathogens in wheat are leaf rust, stripe rust, and powdery mildew. The wheat gene Lr34 has supported resistance to these pathogens for more than 50 years. Lr34 is now shared by wheat cultivars around the world. Here, we show that the LR34 protein resembles adenosine triphosphate-binding cassette transporters of the pleiotropic drug resistance subfamily. Alleles of Lr34 conferring resistance or susceptibility differ by three genetic polymorphisms. The Lr34 gene, which functions in the adult plant, stimulates senescence-like processes in the flag leaf tips and edges.
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            Fast and sensitive silver staining of DNA in polyacrylamide gels

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              Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement

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                Author and article information

                Journal
                Molecular Breeding
                Mol Breeding
                Springer Nature
                1380-3743
                1572-9788
                November 2015
                October 2015
                : 35
                : 11
                Article
                10.1007/s11032-015-0404-4
                © 2015
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