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Abstract
Four closely related chymotrypsin genes were identified in Anopheles aquasalis and
Anopheles darlingi (Anachy1, Anachy2, Andchy1 and Andchy2). The deduced amino-acid
sequences were compared to other chymotrypsin sequences. These sequences were used
to infer phylogenetic relationships among the different species. Genomic cloning revealed
that, in contrast to An. aquasalis and A. gambiae, the chymotrypsin genomic locus
in An. darlingi had a short intergenic region that accompanied the inverted position
of the genes, suggesting inversion mechanisms in this species related to transposable
elements. Alignments of the sequences upstream of the transcription start sites of
Anachy1, Anachy2, Andchy1 and Andchy2 revealed areas with high similarity containing
palindromic sequences. Northern analysis from An. aquasalis indicated that the transcription
of chy 1 and 2 are induced by blood feeding.