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Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots

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      Abstract

      Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological perspective there are benefits in pushing to ∼700 nm to minimize optical absorption (ABS) within tissue and to avoid cell autofluorescence. We report on FRET between streptavidin- (STV-) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated DY731-Bio fluorophores in a donor-acceptor assay. We also highlight the changes in DY731-Bio absorptivity during the streptavidin-biotin binding process which can be attributed to the structural reorientation. For fluorescence beyond 700 nm, different alloy compositions are required for the QD core and these changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with a long-lifetime component in excess of 100 nanoseconds. We compare the influence of the two QD relaxation routes upon FRET dynamics in the presence of DY731-Bio.

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      Most cited references 26

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      Semiconductor Clusters, Nanocrystals, and Quantum Dots

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        (CdSe)ZnS Core−Shell Quantum Dots:  Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites

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          Perspectives on the Physical Chemistry of Semiconductor Nanocrystals

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            Author and article information

            Affiliations
            1School of Physics and Astronomy, Cardiff University, Cardiff, Wales CF24 3AA, UK
            2School of Medicine, Cardiff University, Cardiff, Wales CF14 4XN, UK
            Author notes

            Recommended by Marek Osinski

            Journal
            J Biomed Biotechnol
            JBB
            Journal of Biomedicine and Biotechnology
            Hindawi Publishing Corporation
            1110-7243
            1110-7251
            2007
            12 December 2007
            : 2007
            2217589
            10.1155/2007/54169
            18273410
            Copyright © 2007 E. Z. Chong et al.

            This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

            Categories
            Research Article

            Molecular medicine

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