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      Development of FRET-Based Assays in the Far-Red Using CdTe Quantum Dots

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          Abstract

          Colloidal quantum dots (QDs) are now commercially available in a biofunctionalized form, and Förster resonance energy transfer (FRET) between bioconjugated dots and fluorophores within the visible range has been observed. We are particularly interested in the far-red region, as from a biological perspective there are benefits in pushing to 700 nm to minimize optical absorption (ABS) within tissue and to avoid cell autofluorescence. We report on FRET between streptavidin- (STV-) conjugated CdTe quantum dots, Qdot705-STV, with biotinylated DY731-Bio fluorophores in a donor-acceptor assay. We also highlight the changes in DY731-Bio absorptivity during the streptavidin-biotin binding process which can be attributed to the structural reorientation. For fluorescence beyond 700 nm, different alloy compositions are required for the QD core and these changes directly affect the fluorescence decay dynamics producing a marked biexponential decay with a long-lifetime component in excess of 100 nanoseconds. We compare the influence of the two QD relaxation routes upon FRET dynamics in the presence of DY731-Bio.

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          Semiconductor Clusters, Nanocrystals, and Quantum Dots

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            (CdSe)ZnS Core−Shell Quantum Dots:  Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites

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              Perspectives on the Physical Chemistry of Semiconductor Nanocrystals

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                Author and article information

                Journal
                J Biomed Biotechnol
                JBB
                Journal of Biomedicine and Biotechnology
                Hindawi Publishing Corporation
                1110-7243
                1110-7251
                2007
                12 December 2007
                : 2007
                : 54169
                Affiliations
                1School of Physics and Astronomy, Cardiff University, Cardiff, Wales CF24 3AA, UK
                2School of Medicine, Cardiff University, Cardiff, Wales CF14 4XN, UK
                Author notes

                Recommended by Marek Osinski

                Article
                10.1155/2007/54169
                2217589
                18273410
                b10085c6-9239-49b7-bc3a-c298002b3935
                Copyright © 2007 E. Z. Chong et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 29 March 2007
                : 11 October 2007
                Categories
                Research Article

                Molecular medicine
                Molecular medicine

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