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The Lectin ArtinM Induces Recruitment of Rat Mast Cells from the Bone Marrow to the Peritoneal Cavity

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      Abstract

      Background

      The D-mannose binding lectin ArtinM is known to recruit neutrophils, to degranulate mast cells and may have potential therapeutic applications. However, the effect of ArtinM on mast cell recruitment has not been investigated.

      Methodology

      Male Wistar rats were injected i.p. with ArtinM or ConA (control). The ability of the lectin to degranulate peritoneal and mesenteric mast cells was examined. Recruitment of mast cells to the peritoneal cavity and mesentery after ArtinM injection was examined with or without depletion of peritoneal mast cells by distilled water.

      Results

      ArtinM degranulated both peritoneal and mesentery mast cells in vitro. Three days after i.p. injection of the lectin there were reduced numbers of mast cells in the peritoneal lavage, while at 7 days post injection of ArtinM, the number of peritoneal mast cells was close to control values. Since immature mast cells are recruited from the bone marrow, the effect of the lectin on bone marrow mast cells was examined. Injection of ArtinM resulted in an increased number of mast cells in the bone marrow. To determine if degranulation of mast cells in the peritoneal cavity was required for the increase in bone marrow mast cells, the peritoneal cavity was depleted of mast cells with ultrapure water. Exposure to ArtinM increased the number of mast cells in the bone marrow of rats depleted of peritoneal mast cells.

      Conclusions

      The ArtinM induced recruitment of mast cells from the bone marrow to the peritoneal cavity may partially explain the therapeutic actions of ArtinM.

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      Most cited references 43

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            Author and article information

            Affiliations
            Department of Cell and Molecular Biology and Pathogenic Bioagents, Faculdade de Medicina de Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
            Universidad Nacional, Costa Rica
            Author notes

            Conceived and designed the experiments: PAdAB MRIM DASJ MCJ MCRB CO. Performed the experiments: PAdAB MRIM DASJ CO. Analyzed the data: PAdAB MRIM DASJ MCJ MCRB CO. Contributed reagents/materials/analysis tools: PAdAB MRIM DASJ MCJ MCRB CO. Wrote the paper: PAdAB MRIM DASJ MCJ MCRB CO.

            Contributors
            Role: Editor
            Journal
            PLoS One
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, USA)
            1932-6203
            2010
            22 March 2010
            : 5
            : 3
            2842300
            20339538
            09-PONE-RA-13779R1
            10.1371/journal.pone.0009776
            (Editor)
            Buranello et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
            Counts
            Pages: 11
            Categories
            Research Article
            Cell Biology
            Immunology
            Immunology/Innate Immunity

            Uncategorized

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