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      Release of ATP from Perfused Heart during Coronary Vasodilatation

      ,

      Journal of Vascular Research

      S. Karger AG

      ATP release, Cardiac muscle, Hypoxia, Coronary vasodilatation, Purinergic nerves

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          Abstract

          The release of ATP into the coronary circulation during the vasodilatory response to hypoxia of the perfused guinea pig heart has been measured by the highly sensitive firefly luciferin-luciferase assay method. A reduction in perfusion pressure by more than 50% was accompanied by an increase in ATP concentration in the perfusate from 0.21 ± 0.05 to 0.64 ± 0.09 pmol/ml. When ATP (0.1 µM ) was infused into the coronary circulation, about 99% was bound and/or metabolized during transit, indicating that the measured increase in perfusate ATP during hypoxia is likely to be a substantial underestimate of the true release. This conclusion is supported by experiments with the ATPase inhibitor ouabain, which markedly increased the amount of ATP in the perfusate from the hypoxic heart. Neither guanethidine nor hyoscine significantly altered the vasodilatory response to hypoxia, thus indicating that adrenergic and cholinergic nerves are not responsible. Guanethidine and hyoscine also had no effect on the release of ATP. The possibility that ATP is involved in the physiological regulation of coronary flow is discussed, as well as thequestion of whether the source of the ATP is anoxic cardiac muscle or purinergic nerves.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1974
          1974
          18 September 2008
          : 11
          : 3
          : 110-119
          Affiliations
          Department of Zoology, University of Melbourne, Parkville, Vic.
          Article
          158005 Blood Vessels 1974;11:110–119
          10.1159/000158005
          © 1974 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 10
          Categories
          Research Paper

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