Level of Inositol-1,4,5-Trisphosphate after Cholinergic Stimulation of Human Lymphocytes

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Abstract

The interrelationships between the immune and neurohormonal system are the subject of this work. In this context, the formation of inositol-1,4,5-tris-phosphate (IP3) in response to cholinergic stimulation was studied in activated human peripheral blood mononuclear cells (PBMC) and lymphocytes from the lymphoblastoid cell lines Jurkat and Raji. The cells were stimulated with mitogen and then treated with either the cholinergic agonist carbachol or nicotine. The radioreceptor assay was used to determine IP3. Cholinergic agonists increased the level of IP3in all studied lymphocytes. Maximal level of IP3was observed in PBMC after stimulation with nicotine, whereas in Raji cells IP3level significantly increased after stimulation with carbachol and nicotine. These results indicate that the effect of stimulation of cholinergic receptors present on the activated human lymphocytes appears to be followed by an enhancement in the rate of polyphosphoinositide hydrolysis.

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Journal ID (publisher-id): NIM

Journal ID (nlm-ta): Neuroimmunomodulation

Journal ID (doi): 10.1159/issn.1021-7401

Title: Neuroimmunomodulation

Publisher: S. Karger AG

ISSN (Print): 1021-7401

ISSN (Electronic): 1423-0216

Publication date Subscription-year: 1995

Publication date (Print): 1995

Publication date (Electronic): 22 May 1995

Volume: 2

Issue: 1

Pages: 25-30

Affiliations

Department of Pathophysiology, Institute of Rheumatology, Warsaw, Poland

Article

Publisher ID: 96831 Other ID: Neuroimmunomodulation 1995;2:25–30

DOI: 10.1159/000096831

PubMed ID: 7614257

SO-VID: b1b2f8dd-82b5-4b0e-9e00-fdce6f292723

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Level of Inositol-1,4,5-Trisphosphate after Cholinergic Stimulation of Human Lymphocytes

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Karger: Endocrinology

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