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      Author Correction: Pluripotent stem cell model of Shwachman–Diamond syndrome reveals apoptotic predisposition of hemoangiogenic progenitors

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          Abstract

          Correction to: Scientific Reports 10.1038/s41598-020-71844-8, published online 09 September 2020 This Article contains an error in the Results section, under subheading ‘Apoptotic predisposition of SDS-iPSC–derived hemoangiogenic progenitors.’ “We then investigated the underlying mechanism of the SDS-associated apoptotic predisposition at the hemoangiogenic progenitor stage (Fig. 6a).” should read: “We then investigated the underlying mechanism of the SDS-associated apoptotic predisposition at the hemoangiogenic progenitor stage (Fig. 6a). We confirmed that plasmids encoding shRNA against TP53 were not integrated in all SDS-iPSC clones used in Fig. 6.” In addition, there is an error in the order of the Figures. Figures 2 and 3 are published as Figures 3 and 2 respectively. The correct Figures 2 and 3 appear below as Figures 1 and 2. The Figure legends are correct. Figure 1 Impaired granulopoiesis during in vitro differentiation from SDS-iPSCs. (a) Outline of the defined, step-wise differentiation protocol for generation of mature neutrophils. (b) May–Giemsa staining of floating HCs obtained from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs on day 30 of differentiation. Scale bar: 100 μm. (c) Sequential analysis of the number of floating HCs generated from control SDS-iPSCs, SBDS-overexpressing SDS-iPSCs, and control iPSCs. (d) Sequential analysis of the number of floating HCs generated from SDS-iPSC clones transduced with SBDS or empty vector. (e) Chemotactic activity of floating HCs generated from SDS-iPSC clones transduced with SBDS or empty vector in response to fMLP. fMLP-treated samples were presented as the fold change to untreated control sample. (f) Number of hematopoietic colonies derived from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs. (g) Light micrographs (upper rows) and May–Giemsa staining of hematopoietic colonies (lower rows) generated from SDS-iPSC clones transduced with SBDS or empty vector, and from control iPSCs. Scale bar: 100 μm. Data represent means ± SEM of triplicate wells; representative results from one of three independent experiments are shown (*P < 0.05). Figure 2 Transduction of SDS-iPSCs. (a) Construction of lentiviral vectors containing DsRed alone (PBCl-EF1a-DsRed-PuroR-pA) or SBDS cDNA and DsRed (PBCl-EF1aSBDS-IRES2-DsRed-PuroR-pA). (b) Western blotting analysis of SBDS protein in SDS-iPSC clones transduced with SBDS or empty (DsRed alone) vector. TF2B was used as a loading control. “ + SBDS” indicates iPSC clones transduced with SBDS vector. (c) Representative polysome profiling of SDS-iPSC clones transduced with SBDS or empty vector. (d) 80S:40S ratio in SDS-iPSC clones transduced with SBDS or empty vector. Finally, in the Supplementary Information file, the labels for the original Western blots for Figure 2b are reversed. The correct version of the blots appears below as Figure 3. Figure 3 A correct version of the original Western blots for Figure 2b in the Supplementary Information file.

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          Author and article information

          Contributors
          umeume@kuhp.kyoto-u.ac.jp
          Journal
          Sci Rep
          Sci Rep
          Scientific Reports
          Nature Publishing Group UK (London )
          2045-2322
          18 January 2021
          18 January 2021
          2021
          : 11
          : 2107
          Affiliations
          [1 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Department of Pediatrics, Graduate School of Medicine, , Kyoto University, ; 54 Kawahara-cho, Sakyo-ku, Shogoin, 606-8507 Japan
          [2 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Department of Clinical Application, Center for iPS Cell Research and Application, , Kyoto University, ; Kyoto, 606-8507 Japan
          [3 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Department of Human Health Sciences, Graduate School of Medicine, , Kyoto University, ; Kyoto, 606-8507 Japan
          [4 ]GRID grid.136593.b, ISNI 0000 0004 0373 3971, Department of Cancer Immunotherapy, , Osaka University School of Medicine, ; Suita, 565-0871 Japan
          [5 ]GRID grid.261356.5, ISNI 0000 0001 1302 4472, Department of Pediatric Hematology/Oncology, , Okayama University, ; Okayama, 700-8558 Japan
          [6 ]GRID grid.267335.6, ISNI 0000 0001 1092 3579, Department of Pediatrics, Graduate School of Biomedical Sciences, , Tokushima University, ; Tokushima, 770-8501 Japan
          [7 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, , Kyoto University, ; Kyoto, 606-8507 Japan
          [8 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Department of Life Science Frontiers, Center for iPS Cell Research and Application, , Kyoto University, ; Kyoto, 606-8507 Japan
          [9 ]GRID grid.258799.8, ISNI 0000 0004 0372 2033, Drug Discovery Technology Development Office, Center for iPS Cell Research and Application, , Kyoto University, ; Kyoto, 606-8507 Japan
          [10 ]GRID grid.415798.6, ISNI 0000 0004 0378 1551, Department of Hematology and Oncology, , Shizuoka Children’s Hospital, ; Shizuoka, 420-8660 Japan
          Author information
          http://orcid.org/0000-0002-2932-4960
          http://orcid.org/0000-0001-8813-3614
          http://orcid.org/0000-0001-7238-2763
          http://orcid.org/0000-0001-5806-1090
          Article
          81066
          10.1038/s41598-021-81066-1
          7814114
          33462257
          b1b35f73-e681-471e-87db-5c66dc0e28e7
          © The Author(s) 2021

          Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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