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      A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

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          Abstract

          Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

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          Author and article information

          Contributors
          cfleong@ppukm.ukm.edu.my
          Journal
          Indian J Hematol Blood Transfus
          Indian J Hematol Blood Transfus
          Indian Journal of Hematology & Blood Transfusion
          Springer India (New Delhi )
          0971-4502
          0974-0449
          21 August 2019
          January 2020
          : 36
          : 1
          : 112-116
          Affiliations
          [1 ]Department of Pathology, Hospital Pekan, Pekan, Pahang Darul Makmur Malaysia
          [2 ] GRID grid.240541.6, ISNI 0000 0004 0627 933X, Department of Pathology, Faculty of Medicine, , Universiti Kebangsaan Malaysia Medical Centre, ; Kuala Lumpur, Malaysia
          [3 ] GRID grid.240541.6, ISNI 0000 0004 0627 933X, Department of Medical Microbiology and Immunology, , Universiti Kebangsaan Malaysia Medical Centre, ; Kuala Lumpur, Malaysia
          [4 ] GRID grid.412516.5, ISNI 0000 0004 0621 7139, Department of Pathology, , Hospital Kuala Lumpur, ; Kuala Lumpur, Malaysia
          Author information
          http://orcid.org/0000-0001-8403-9866
          Article
          PMC7042476 PMC7042476 7042476 1171
          10.1007/s12288-019-01171-0
          7042476
          32174694
          b1c4a2d3-32f6-4964-9a3e-5672f5beea18
          © Indian Society of Hematology and Blood Transfusion 2019
          History
          : 5 May 2019
          : 13 August 2019
          Funding
          Funded by: UKMMC Fundamental Research Fund
          Award ID: FF-2016-075
          Award Recipient :
          Categories
          Original Article
          Custom metadata
          © Indian Society of Hematology and Blood Transfusion 2020

          Sterility,Coagulation parameters,Refrozen,Thawed fresh frozen plasma

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