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      Preparation, characterisation and cell transfection of cationic liposomes in gene therapy

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      British Journal of Pharmacy
      University of Huddersfield Press

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          Abstract

          Cationic lipid-mediated gene transfer is one of the most commonly used non-viralvectors. It has been shown to be a safe and effective carrier. However, its use ingene delivery was hampered by its low transfection efficiency and stability.DOTAP, DOPE, cholesterol (CHO) and carboxymethyl-β-cyclodextrin (CD) wereused to prepare cationic liposomes. Cationic liposomes were prepared using both,thin film hydration and a microfluidic method. Formulation stability wasevaluated using liposome size, zeta potential and polydispersity index (PDI).Promega QuantiFluor® ONE dsDNA System was used to investigate theencapsulation efficiency. COS7 and SH-SY5Y cell lines were used to determinetransfection efficiency. Results show that carboxymethyl-β-cyclodextrin increasedencapsulation efficiency by 15.5% and 8% using NanoAssemblr® and rotaryevaporator, respectively compared to liposomes without CD. The addition ofcarboxymethyl-β-cyclodextrin to cationic liposomes resulted in an increase intransfection efficiency in both cell lines.B

          Author and article information

          Journal
          British Journal of Pharmacy
          University of Huddersfield Press
          2058-8356
          April 17 2019
          April 17 2019
          : 4
          : 1
          Article
          10.5920/bjpharm.618
          b1e00505-df0c-4e41-a6e0-f4fcc9d3fd37
          © 2019

          This work is licensed under a Creative Commons Attribution 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

          History

          Medicine,Pharmacology & Pharmaceutical medicine,Health & Social care
          Medicine, Pharmacology & Pharmaceutical medicine, Health & Social care

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