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      Antisense mRNA for NPY-Y1 receptor in the medial preoptic area increases prolactin secretion

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          Abstract

          We investigated the participation of neuropeptide Y-Y1 receptors within the medial preoptic area in luteinizing hormone, follicle-stimulating hormone and prolactin release. Four bilateral microinjections of sense (control) or antisense 18-base oligonucleotides of messenger ribonucleic acid (mRNA) (250 ng) corresponding to the NH2-terminus of the neuropeptide Y1 receptor were performed at 12-h intervals for two days into the medial preoptic area of ovariectomized Wistar rats (N = 16), weighing 180 to 200 g, treated with estrogen (50 µg) and progesterone (25 mg) two days before the experiments between 8.00 and 10:00 a.m. Blockade of Y1 receptor synthesis in the medial preoptic area by the antisense mRNA did not change plasma luteinizing hormone or follicle-stimulating hormone but did increase prolactin from 19.6 ± 5.9 ng/ml in the sense group to 52.9 ± 9.6 ng/ml in the antisense group. The plasma hormones were measured by radioimmunoassay and the values are reported as mean ± SEM. These data suggest that endogenous neuropeptide Y in the medial preoptic area has an inhibitory action on prolactin secretion through Y1 receptors.

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          Most cited references 47

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          Neuropeptide Y--a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide.

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            The anatomy of neuropeptide-Y-containing neurons in rat brain.

            The distribution of neuropeptide Y in the central nervous system of adult male rats was investigated using indirect immunofluorescence, the peroxidase-antiperoxidase technique and by radioimmunoassay of microdissected brain regions. The different methods were in good agreement and showed that neuropeptide Y had a widespread distribution and was present in extremely high concentrations. The highest concentrations of neuropeptide Y were found in the paraventricular hypothalamic nucleus and hypothalamic arcuate nucleus, which also contained the highest density of immunoreactive fibers and numbers of perikarya, respectively. The suprachiasmatic nucleus, median eminence, dorsomedial hypothalamic nucleus and paraventricular thalamic nucleus showed high concentrations as well as high densities of fibers. Moderate concentrations were found in the bed nucleus of the stria terminalis, although a high density of fibers was found. Areas with moderate concentrations and densities of fibers were the medial preoptic area, anterior hypothalamic area, periventricular nucleus, posterior hypothalamus and the medial amygdaloid nucleus. The nucleus of the solitary tract contained a low concentration of neuropeptide Y although a high number of immunoreactive perikarya was found in colchicine-treated rats. Low concentrations were also measured in the cerebral cortex, yet relatively high numbers of cell bodies and fibers were found dispersed through the cortex. The extremely high concentrations and widespread distribution of neuropeptide Y in the central nervous system suggests a number of important physiological roles for this neurotransmitter candidate.
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              Mandatory neuropeptide-steroid signaling for the preovulatory luteinizing hormone-releasing hormone discharge

               S P Kalra (1993)
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                Author and article information

                Journal
                bjmbr
                Brazilian Journal of Medical and Biological Research
                Braz J Med Biol Res
                Associação Brasileira de Divulgação Científica (Ribeirão Preto, SP, Brazil )
                0100-879X
                1414-431X
                September 1999
                : 32
                : 9
                : 1161-1165
                Affiliations
                Goiânia GO orgnameUniversidade de Goiás orgdiv1 Departamento de Fisiologia, Instituto de Ciências Biológicas Brasil
                Ribeirão Preto SP orgnameUniversidade de São Paulo orgdiv1 Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto Brasil
                Article
                S0100-879X1999000900016 S0100-879X(99)03200916
                10.1590/S0100-879X1999000900016

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 30, Pages: 5
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                Categories
                Physiology and biophysics

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