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      Membrane Mechanism Mediates Progesterone Stimulatory Effect on LHRH Release from Superfused Rat Hypothalami in vitro



      S. Karger AG

      Immobilized steroids, LHRH, Progesterone, Hypothalamus

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          To determine whether the plasma membrane is a primary site for progesterone (P4) action on the neural LHRH apparatus of hypothalamic tissues derived from ovariectomized, estradiol-primed (OVX + E<sub>2</sub>) immature rats, immobilized P4 was infused directly to these tissues using a superfusion technique. Two kinds of immobilized P4 with bovine serum albumin (BSA) conjugated at positions 3 or 11, or 11-deoxycorticosterone (DOC) immobilized at position 21 of the steroid molecule, respectively, were tested for structural specificity. Among the three immobilized steroids, only P<sub>4</sub> with BSA conjugated at position 3 (P4–3-BSA) was effective in stimulating LHRH release in vitro. P<sub>4</sub>-3-BSA at 0.5 µg/ml, approximately 1.7 × 10<sup>–7</sup> M of P4, increased LHRH levels in the superfusates to about 2.5-fold those of pretreatment levels. In addition, no conversion of P4–3-BSA to free progesterone was detected. This observation demonstrated that the plasma membrane is a primary site for the stimulating effect of P4 on LHRH release from hypothalamic tissue in vitro.

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          Author and article information

          S. Karger AG
          02 April 2008
          : 45
          : 6
          : 514-517
          Department of Physiology and Biophysics, University of Illinois at Urbana-Champaign, Urbana, Ill. USA
          124784 Neuroendocrinology 1987;45:514–517
          © 1987 S. Karger AG, Basel

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