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      Overloaded and stressed: whole-cell considerations for bacterial synthetic biology.

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          Abstract

          The predictability and robustness of engineered bacteria depend on the many interactions between synthetic constructs and their host cells. Expression from synthetic constructs is an unnatural load for the host that typically reduces growth, triggers stresses and leads to decrease in performance or failure of engineered cells. Work in systems and synthetic biology has now begun to address this through new tools, methods and strategies that characterise and exploit host-construct interactions in bacteria. Focusing on work in E. coli, we review here a selection of the recent developments in this area, highlighting the emerging issues and describing the new solutions that are now making the synthetic biology community consider the cell just as much as they consider the construct.

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          Author and article information

          Journal
          Curr. Opin. Microbiol.
          Current opinion in microbiology
          Elsevier BV
          1879-0364
          1369-5274
          Oct 2016
          : 33
          Affiliations
          [1 ] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK.
          [2 ] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK. Electronic address: g.stan@imperial.ac.uk.
          [3 ] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Department of Bioengineering, Imperial College London, London, UK. Electronic address: t.ellis@imperial.ac.uk.
          Article
          S1369-5274(16)30097-2
          10.1016/j.mib.2016.07.009
          27494248
          b2632a43-c1ec-4ca0-97ca-b944cc5a4b69
          History

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