Blog
About

3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Shigella subverts the host recycling compartment to rupture its vacuole.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Shigella enters epithlial cells via internalization into a vacuole. Subsequent vacuolar membrane rupture allows bacterial escape into the cytosol for replication and cell-to-cell spread. Bacterial effectors such as IpgD, a PI(4,5)P2 phosphatase that generates PI(5)P and alters host actin, facilitate this internalization. Here, we identify host proteins involved in Shigella uptake and vacuolar membrane rupture by high-content siRNA screening and subsequently focus on Rab11, a constituent of the recycling compartment. Rab11-positive vesicles are recruited to the invasion site before vacuolar rupture, and Rab11 knockdown dramatically decreases vacuolar membrane rupture. Additionally, Rab11 recruitment is absent and vacuolar rupture is delayed in the ipgD mutant that does not dephosphorylate PI(4,5)P₂ into PI(5)P. Ultrastructural analyses of Rab11-positive vesicles further reveal that ipgD mutant-containing vacuoles become confined in actin structures that likely contribute to delayed vacular rupture. These findings provide insight into the underlying molecular mechanism of vacuole progression and rupture during Shigella invasion.

          Related collections

          Author and article information

          Journal
          Cell Host Microbe
          Cell host & microbe
          1934-6069
          1931-3128
          Oct 8 2014
          : 16
          : 4
          Affiliations
          [1 ] Institut Pasteur, Dynamics of Host-Pathogen interactions Unit, 25 Rue du Dr. Roux, 75724 Paris, France.
          [2 ] Institut Pasteur, Imagopole, 28 Rue du Dr. Roux, 75724 Paris, France.
          [3 ] Department of Materials and Interfaces, Weizmann Institute of Sciences, 234 Herzl Street, Rehovot 76100, Israel.
          [4 ] Institut Pasteur, Imagopole, 28 Rue du Dr. Roux, 75724 Paris, France. Electronic address: anne.danckaert@pasteur.fr.
          [5 ] Institut Pasteur, Dynamics of Host-Pathogen interactions Unit, 25 Rue du Dr. Roux, 75724 Paris, France. Electronic address: jost.enninga@pasteur.fr.
          Article
          S1931-3128(14)00338-2
          10.1016/j.chom.2014.09.005
          25299335
          Copyright © 2014 Elsevier Inc. All rights reserved.

          Comments

          Comment on this article