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      OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes

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          Abstract

          Background

          Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice drought resistance have not been characterized.

          Results

          In this study, a novel GRAS transcription factor gene named OsGRAS23, which is located in a drought-resistant QTL interval on chromosome 4 of rice, was isolated. The expression of OsGRAS23 was induced by drought, NaCl, and jasmonic acid treatments. The OsGRAS23-GFP fused protein was localized in the nucleus of tobacco epidermal cells. A trans-activation assay in yeast cells demonstrated that the OsGRAS23 protein possessed a strong transcriptional activation activity. OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H 2O 2 accumulation compared with the wild-type plants. Furthermore, microarray analysis showed that several anti-oxidation related genes were up-regulated in the OsGRAS23-overexpressing rice plants. The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

          Conclusions

          Our results demonstrate that OsGRAS23 encodes a stress-responsive GRAS transcription factor and positively modulates rice drought tolerance via the induction of a number of stress-responsive genes.

          Electronic supplementary material

          The online version of this article (doi:10.1186/s12870-015-0532-3) contains supplementary material, which is available to authorized users.

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          OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression.

          Joseph G Dubouzet, Yoh Sakuma, Yusuke Ito … (2003)
          The transcription factors DREBs/CBFs specifically interact with the dehydration-responsive element/C-repeat (DRE/CRT) cis-acting element (core motif: G/ACCGAC) and control the expression of many stress-inducible genes in Arabidopsis. In rice, we isolated five cDNAs for DREB homologs: OsDREB1A, OsDREB1B, OsDREB1C, OsDREB1D, and OsDREB2A. Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over-expression of OsDREB1A in transgenic Arabidopsis induced over-expression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high-salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis. The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1-type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses.
          Article 0 comments Cited 436 times – based on 0 reviews     Review now
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            Research on plant abiotic stress responses in the post-genome era: past, present and future.

            Takashi HIRAYAMA, Kazuo Shinozaki (2010)
            Understanding abiotic stress responses in plants is an important and challenging topic in plant research. Physiological and molecular biological analyses have allowed us to draw a picture of abiotic stress responses in various plants, and determination of the Arabidopsis genome sequence has had a great impact on this research field. The availability of the complete genome sequence has facilitated access to essential information for all genes, e.g. gene products and their function, transcript levels, putative cis-regulatory elements, and alternative splicing patterns. These data have been obtained from comprehensive transcriptome analyses and studies using full-length cDNA collections and T-DNA- or transposon-tagged mutant lines, which were also enhanced by genome sequence information. Moreover, studies on novel regulatory mechanisms involving use of small RNA molecules, chromatin modulation and genomic DNA modification have enabled us to recognize that plants have evolved complicated and sophisticated systems in response to complex abiotic stresses. Integrated data obtained with various 'omics' approaches have provided a more comprehensive picture of abiotic stress responses. In addition, research on stress responses in various plant species other than Arabidopsis has increased our knowledge regarding the mechanisms of plant stress tolerance in nature. Based on this progress, improvements in crop stress tolerance have been attempted by means of gene transfer and marker-assisted breeding. In this review, we summarize recent progress in abiotic stress studies, especially in the post-genomic era, and offer new perspectives on research directions for the next decade.
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              Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways.

              K Shinozaki, K Yamaguchi-Shinozaki (2000)
              Recently, a major transcription system that controls abscisic-acid-independent gene expression in response to dehydration and low temperature has been identified. The system includes the DRE/CRT (dehydration-responsive element/C-repeat) cis-acting element and its DNA-binding protein, DREB/CBF (DRE-binding protein/C-repeat binding factor), which has an AP2 domain. DREB/CBF contains two subclasses, DREB1/CBF and DREB2, which are induced by cold and dehydration, respectively, and control the expression of various genes involved in stress tolerance. Recent studies are providing evidence of differences between dehydration-signaling and cold-stress-signaling cascades, and of cross-talk between them.
              Article 0 comments Cited 333 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Kai Xu: kxu@sagc.org.cn
                Shoujun Chen: csj08@sagc.org.cn
                Tianfei Li: ltf@sagc.org.cn
                Xiaosong Ma: mxs09@sagc.org.cn
                Xiaohua Liang: lxh@sagc.org.cn
                Xuefeng Ding: dxf07@sagc.org.cn
                Hongyan Liu: lhy@sagc.org.cn
                Lijun Luo: lijun@sagc.org.cn
                Journal
                Journal ID (nlm-ta): BMC Plant Biol
                Journal ID (iso-abbrev): BMC Plant Biol
                Title: BMC Plant Biology
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1471-2229
                Publication date (Electronic): 13 June 2015
                Publication date PMC-release: 13 June 2015
                Publication date Collection: 2015
                Volume: 15
                Electronic Location Identifier: 141
                Affiliations
                [ ]Shanghai Agrobiological Gene Center, Shanghai, 201106 China
                [ ]Huazhong Agriculture University, Wuhan, 430070 China
                Article
                Publisher ID: 532
                DOI: 10.1186/s12870-015-0532-3
                PMC ID: 4465154
                PubMed ID: 26067440
                SO-VID: b2df5cf3-3750-4f4e-a071-43c87dea36af
                Copyright © © Xu et al. 2015
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

                History
                Date received : 15 December 2014
                Date accepted : 21 May 2015
                Categories
                Subject: Research Article
                Custom metadata
                issue-copyright-statement © The Author(s) 2015

                ScienceOpen disciplines: Plant science & Botany
                Keywords: drought resistance,gras,rice,transcription factor
                Data availability:
                ScienceOpen disciplines: Plant science & Botany
                Keywords: drought resistance, gras, rice, transcription factor

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