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      Relación entre el recuento de células somáticas individual o en tanque de leche y la prueba CMT en dos fincas lecheras del departamento de Antioquia (Colombia) Translated title: Relationship between individual and bull tank somatic cell counts with CMT test in two dairy herds in Antioquia (Colombia)

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          Abstract

          El objetivo del presente proyecto fue establecer relaciones entre la prueba de CMT y los recuentos de células somáticas individuales o en el tanque de la leche, con el objetivo de definir un modelo predictivo del número de células somáticas en el tanque y proponer un modelo para detectar fincas con mastitis subclínica. En dos fincas lecheras del Municipio de San Pedro de los Milagros (Colombia), se tomaron muestras de leche de las vacas en ordeño (n = 95) durante los ordeños de la mañana y de la tarde (una muestra/mes/3 meses). En el ordeño de la tarde se realizó prueba de CMT y en todos los muestreos se hizo recuento de células somáticas en el medidor individual (RCS), y en el tanque de la leche, usando un equipo Fossomatic 90. Para el análisis estadístico se realizó una transformación logarítmica de los datos (y = Log2(RCS/100) +3), para ajustarlos a la normalidad y realizar análisis de varianza. Los resultados mostraron una relación estadística significativa (p<0.05) entre el porcentaje de cuartos afectados por mastitis subclínica según la prueba de CMT y el BTSCC del ordeño de la tarde. Además, se definió una fórmula predictiva para el BTSCC de acuerdo con el porcentaje de cuartos afectados para el ordeño de la tarde. Los resultados indicaron un promedio de RCS de 206.630 cel/ml y 145.935 cel/ml para los ordeños de la tarde y la mañana, respectivamente; mientras que el BTSCC presentó un promedio de 186.830 cel/ml y 93.145 cel/ml, para los ordeños de la tarde y la mañana, respectivamente. Asimismo, se halló un relación estadística significativa (p<0.05) entre el RCS del ordeño de la tarde con el BTSCC. Los resultados del BTSCC se encuentran por debajo de los valores límites permitidos en Europa y Estados Unidos, lo cual sugiere que bajo condiciones de manejo estrictas, las fincas lecheras en Antioquia pueden lograr los estándares internacionales de calidad para la exportación de leche. Estudios adicionales se requieren para precisar las variaciones en las relaciones entre los recuentos individuales y del tanque, hallados para los ordeños de la mañana y de la tarde.

          Translated abstract

          In order to establish a mathematical model with which to predict the Bull Tank Somatic Cell Count (BTSCC) of herds with sub clinical mastitis and to search for possible relationships between CMT results and individual somatic cell counts (SCC) or BTSCC, a descriptive study was carried out in two farms located in the dairy region of Antioquia (Colombia), in which lactating dairy cows (n = 95) were sampled during the morning and afternoon milking (once a month/3 months). CMT evaluation was performed at the afternoon milking at each time point of evaluation. In addition, total milk produced by individual cows was recorded and a milk sample was taken to perform SCC. Similarly, three samples of milk were taken from the tank to measure BTSCC. All milk samples were processed by triplicates by using a Fossomatic-90 equipment. Logarithmic transformation of data were done to normalize the SCC and BTSCC results according to the model: logarithmic SCC (SCCL) = Log2(SCC/100) +3, and analysis of variance were performed. A significant relationship (p<0.05) was found between the percent of positive quarters (at least one cross by CMT) and the BTSCC taken at the afternoon milking. Accordingly, a model was established to define the BTSCC value depending on the percentage of CMT positive quarters. The average SCC of 206.630 and 145.935 cel/ml, were found for afternoon and morning milking, respectively; in as much as the average BTSCC found were 186.830 and 93.145 cel/ml, for afternoon and morning milking, respectively. Furthermore, a significant relationship (p<0.05) was found between the SCC of the afternoon milking and the BTSCC. The BTSCC values were lower than the limit values accepted for the United Sates and European countries, which suggest that under strictly controlled management policies the dairy herds from Antioquia could meet the international standards for milk exportation. Finally, further studies are required in order to precise and define the source of variations found between the SCC and BTSCC for both the morning and afternoon milking.

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          Most cited references23

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          Physiology of mastitis and factors affecting somatic cell counts.

          Inflammation of the mammary gland that results from the introduction and multiplication of pathogenic microorganisms in the mammary gland is a complex series of events leading to reduced synthetic activity, compositional changes, and elevated SCC. The magnitude and temporal relationships of these responses vary with nutritional status, other animal factors, and the pathogen involved. Because the elevation of SCC is a response to an insult to the mammary gland and is modulated by inflammatory mediators, the major factor influencing SCC is infection status. The effects of stage of lactation, age, season, and various stresses on SCC are minor if the gland is uninfected. Except for normal diurnal variation, few factors other than infection status have a significant impact on milk SCC.
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            Low somatic cell count: a risk factor for subsequent clinical mastitis in a dairy herd.

            A case-control study was conducted to evaluate factors measured at the udder inflammation-free state as risk factors for subsequent clinical mastitis. The factors including somatic cell count (SCC), body condition score, milk yield, percentages of milk fat and milk protein, and diseases were evaluated for their association with the results of udder inflammatory response. The results of the response were specified as presence (case) and absence (control) of clinical signs of mastitis. Data on Holstein Friesian cows calving from January 1984 to November 1996 from a commercial farm with low bulk milk SCC were used. Univariable and multivariable random-effect logistic models were used to evaluate the effect of those factors on the risk of clinical mastitis. The following variables were associated with increased odds of case versus control events in the univariable analysis: early lactation period, low SCC, high milk yield, high percentage of milk protein, high percentage of milk fat, low body condition score, retained placenta, and milk fever. For the final multivariable model of all variables used for analysis, only low SCC remained significantly associated with increased risk of subsequent clinical mastitis. The authors concluded that very low SCC during the udder inflammation-free state are associated with increased risk of clinical mastitis.
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              Estimation of variance components for somatic cell counts to determine thresholds for uninfected quarters.

              The objective of this study was to determine the factors affecting somatic cell count (SCC), to estimate variance components of these factors, and to calculate and evaluate the thresholds for intramammary infection based on SCC. The infection status from 22,467 quarter milk samples from 544 cows in seven herds was determined. Infections status was the most important factor affecting SCC. The increase in SCC was more pronounced for major pathogens than for minor pathogens. Even after adjustment for infection status, the interaction between stage of lactation and parity was significant. For culture-negative samples within a lactation, the shape of the SCC curve was inversely related to the shape of the milk production curve. The shape of the SCC curve was flat for first lactation cows compared with the shape of the SCC curve for cows in subsequent lactations. The effect of clinical mastitis on SCC was significant. The use of SCC thresholds for specific parities and stages of lactation to detect intramammary infection improved quality parameters only slightly over a fixed threshold of 200,000 cells/ml.
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                Author and article information

                Journal
                rccp
                Revista Colombiana de Ciencias Pecuarias
                Rev Colom Cienc Pecua
                Facultad de Ciencias Agrarias, Universidad de Antioquia (Medellín, Antioquia, Colombia )
                0120-0690
                2256-2958
                December 2007
                : 20
                : 4
                : 472-483
                Affiliations
                [02] Medellín Colombia orgnameUniversidad de Antioquia orgdiv1Facultad de Ciencias Agrarias orgdiv2Escuela de Medicina veterinaria mceronm@ 123456agronica.udea.edu.co
                [01] Medellín orgnameUniversidad de Antioquia orgdiv1Facultad de Ciencias Agrarias orgdiv2Grupo de investigación en genética molecular y mejoramiento anima Colombia
                Article
                S0120-06902007000400006 S0120-0690(07)02000406
                b2ecba7e-35e8-4a67-99f0-608d95440ffb

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

                History
                : 25 August 2006
                : 13 September 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 27, Pages: 12
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Articulos Originales

                bovine,BON x Holstein cows,holstein cows,milk quality,somatic cell counts,bovinos,calidad de la leche,recuento de células somáticas,vacas BON x holstein,vacas holstein

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