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      ScRALF3, a secreted RALF-like peptide involved in cell-cell communication between the sporophyte and the female gametophyte in a solanaceous species.

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          Abstract

          Small peptides have been shown to regulate numerous aspects of plant development through cell-cell communication. These signaling events are particularly important during reproduction, regulating gamete development and embryogenesis. Rapid alkalinization factor (RALF)-like genes, a large gene family that encodes secreted peptides, have specific or ubiquitous expression patterns. Previously, five RALF-like genes with potential involvement during reproduction were isolated from Solanum chacoense. Here, we show that ScRALF3 is an important peptide regulator of female gametophyte development. Its expression, which is auxin-inducible, is strictly regulated before and after fertilization. Down-regulation of ScRALF3 expression by RNA interference leads to the production of smaller fruits that produce fewer seeds, due to improper development of the embryo sacs. Defects include loss of embryo sac nuclei polarization, as well as an increase in asynchronous division, accounting for cellular dysfunctions and premature embryo sac development arrest during megagametogenesis. ScRALF3 is expressed in the sporophytic tissue surrounding the embryo sac, the integument and the nucellus, as revealed by in situ hybridization and GUS staining. As expected for a secreted peptide, fluorescence from an ScRALF3-GFP fusion construct is detected throughout the secretory pathway. Therefore, the ScRALF3 secreted peptide may be directly involved in the regulation of multiple aspects of cell-cell communication between the female gametophyte and its surrounding sporophytic tissue during ovule development.

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          Author and article information

          Journal
          Plant J.
          The Plant journal : for cell and molecular biology
          Wiley-Blackwell
          1365-313X
          0960-7412
          Mar 2013
          : 73
          : 6
          Affiliations
          [1 ] Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques Université de Montréal, 4101 rue Sherbrooke est, Montréal, Québec, H1X 2B2, Canada.
          Article
          10.1111/tpj.12096
          23237060
          b303df13-1b18-4798-a1b2-ed6b5aa93189
          History

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