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      The BeWo choriocarcinoma cell line as a model of iodide transport by placenta

      , ,
      Placenta
      Elsevier BV

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          Abstract

          Cultured human choriocarcinoma cells of the BeWo line exhibited saturable accumulation of radioiodide. Inhibition by competing anions followed the affinity series perchlorate > iodide > or = thiocyanate, consistent with uptake through the thyroid iodide transporter, NIS, whose messenger RNA was found in BeWo cells, and whose protein was distributed towards the apical pole of the cells. Efflux obeyed first order kinetics and was inhibited by DIDS, an antagonist of anion exchangers including pendrin, whose messenger RNA was also present. In cultures where iodide uptake through NIS was blocked with excess perchlorate, radioiodide accumulation was stimulated by exposure to medium in which physiological anions were replaced by 2-morpholinoethanesulfonic acid (MES), consistent with the operation of an anion exchange mechanism taking up iodide. Chloride in the medium was more effective than sulfate at inhibiting this uptake, matching the ionic specificity of pendrin. These studies provide evidence that the trophoblast accumulates iodide through NIS and releases it to the fetal compartment through pendrin.

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          Author and article information

          Journal
          Placenta
          Placenta
          Elsevier BV
          01434004
          May 2005
          May 2005
          : 26
          : 5
          : 380-386
          Article
          10.1016/j.placenta.2004.07.004
          15850642
          b369ca17-699c-4ae2-b874-044fdcb6b58f
          © 2005

          http://www.elsevier.com/tdm/userlicense/1.0/

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