ScienceOpen:
research and publishing network
For Publishers
Discovery
Metadata
Peer review
Hosting
Publishing
For Researchers
Join
Publish
Review
Collect
My ScienceOpen
Sign in
Register
Dashboard
Blog
About
Search
Advanced search
My ScienceOpen
Sign in
Register
Dashboard
Search
Search
Advanced search
For Publishers
Discovery
Metadata
Peer review
Hosting
Publishing
For Researchers
Join
Publish
Review
Collect
Blog
About
50
views
0
references
Top references
cited by
80
Cite as...
0 reviews
Review
0
comments
Comment
0
recommends
+1
Recommend
0
collections
Add to
0
shares
Share
Twitter
Sina Weibo
Facebook
Email
2,081
similar
All similar
Record
: found
Abstract
: not found
Article
: not found
Fungal secondary metabolites – Strategies to activate silent gene clusters
Author(s):
Axel A. Brakhage
,
Volker Schroeckh
Publication date
Created:
January 2011
Publication date
(Print):
January 2011
Journal:
Fungal Genetics and Biology
Publisher:
Elsevier BV
Read this article at
ScienceOpen
Publisher
PubMed
Review
Review article
Invite someone to review
Bookmark
Cite as...
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Filamentous fungi produce a multitude of low molecular weight bioactive compounds. The increasing number of fungal genome sequences impressively demonstrated that their biosynthetic potential is far from being exploited. In fungi, the genes required for the biosynthesis of a secondary metabolite are clustered. Many of these bioinformatically newly discovered secondary metabolism gene clusters are silent under standard laboratory conditions. Consequently, no product can be found. This review summarizes the current strategies that have been successfully applied during the last years to activate these silent gene clusters in filamentous fungi, especially in the genus Aspergillus. The techniques take advantage of genome mining, vary from the simple search for compounds with bioinformatically predicted physicochemical properties up to methods that exploit a probable interaction of microorganisms. Until now, the majority of successful approaches have been based on molecular biology like the generation of gene "knock outs", promoter exchange, overexpression of transcription factors or other pleiotropic regulators. Moreover, strategies based on epigenetics opened a new avenue for the elucidation of the regulation of secondary metabolite formation and will certainly continue to play a significant role for the elucidation of cryptic natural products. The conditions under which a given gene cluster is naturally expressed are largely unknown. One technique is to attempt to simulate the natural habitat by co-cultivation of microorganisms from the same ecosystem. This has already led to the activation of silent gene clusters and the identification of novel compounds in Aspergillus nidulans. These simulation strategies will help discover new natural products in the future, and may also provide fundamental new insights into microbial communication. Copyright © 2010 Elsevier Inc. All rights reserved.
Related collections
CRISPR/Cas9 editing in human blood
Author and article information
Journal
Title:
Fungal Genetics and Biology
Abbreviated Title:
Fungal Genetics and Biology
Publisher:
Elsevier BV
ISSN (Print):
10871845
Publication date Created:
January 2011
Publication date (Print):
January 2011
Volume
: 48
Issue
: 1
Pages
: 15-22
Article
DOI:
10.1016/j.fgb.2010.04.004
PubMed ID:
20433937
SO-VID:
b36d5ef9-d081-4c90-b40e-355bd5de2b10
Copyright ©
© 2011
License:
https://www.elsevier.com/tdm/userlicense/1.0/
History
Data availability:
Comments
Comment on this article
Sign in to comment
scite_
Similar content
2,081
Students' Silent Messages: Can Teacher Verbal and Nonverbal Immediacy Moderate Student Use of Text Messaging in Class?
Authors:
Fang-Yi Flora Wei
,
Y. Ken Wang
The cannabinoid 1-receptor silent antagonist O-2050 attenuates preference for high-fat diet and activated astrocytes in mice.
Authors:
Katsunori Iwasaki
,
Yoshiharu Akitake
,
Sei Higuchi
…
Silent strain of caregiving: exploring the best predictors of distress in family carers of geriatric patients
Authors:
Katarzyna Bień-Barkowska
,
Halina Doroszkiewicz
,
Barbara Bień
See all similar
Cited by
197
The amazing potential of fungi: 50 ways we can exploit fungi industrially
Authors:
Kevin D. Hyde
,
Jianchu Xu
,
Sylvie Rapior
…
Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes
Authors:
Florence Mus
,
Matthew Crook
,
Kevin Garcia
…
Co-Cultivation—A Powerful Emerging Tool for Enhancing the Chemical Diversity of Microorganisms
Authors:
Andreas Marmann
,
Amal Aly
,
Wenhan Lin
…
See all cited by
Version 1
- Current
Version 1