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      Ultra-high resolution crystal structure of recombinant caprine β-lactoglobulin.

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          Abstract

          β-Lactoglobulin (βlg) is the most abundant whey protein in the milks of ruminant animals. While bovine βlg has been subjected to a vast array of studies, little is known about the caprine ortholog. We present an ultra-high resolution crystal structure of caprine βlg complemented by analytical ultracentrifugation and small-angle X-ray scattering data. In both solution and crystalline states caprine βlg is dimeric (K(D)<5 μM); however, our data suggest a flexible quaternary arrangement of subunits within the dimer. These structural findings will provide insight into relationships among structural, processing, nutritional and immunological characteristics that distinguish cow's and goat's milk.

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          Author and article information

          Journal
          FEBS Lett.
          FEBS letters
          1873-3468
          0014-5793
          Nov 3 2014
          : 588
          : 21
          Affiliations
          [1 ] Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
          [2 ] Institute of Fundamental Sciences and The Riddet Institute, Massey University, Palmerston North, New Zealand.
          [3 ] Food and Bio-based Products, AgResearch Limited, Ruakura Research Centre, Hamilton, New Zealand.
          [4 ] Biomolecular Interaction Centre, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: renwick.dobson@canterbury.ac.nz.
          Article
          S0014-5793(14)00685-1
          10.1016/j.febslet.2014.09.010
          25241165
          b39e1521-54ae-49c0-8c02-22a9880cf85e
          Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
          History

          Analytical ultracentrifugation,Caprine,Goat,Milk whey,Small-angle X-ray scattering,Ultra-high resolution structure

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