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      Regulation and Physiological Role of Insulin-Like-Growth-Factor-Binding Protein-1 in Human Granulosa Cells

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          Abstract

          The present study was undertaken to elucidate the physiological role and the regulation of insulin-like growth factor (IGF)-binding protein-1 (IGFBP-1) in human luteinizing granulosa cells. IGFBP-1 abolished IGF-I-stimulated estradiol production by luteinizing granulosa cells in a dose-dependent manner with a 50% inhibitory concentration (IC<sub>50</sub>) of 0.27 n M. Similarly, IGFBP-1 inhibited <sup>125</sup>I-IGF-I binding to granulosa cells. IGFBP-1 was identified by Western immunoblot and specific enzyme immunoassay in the granulosa-cell-conditioned medium after 24 h culture. Immunoreactive IGFBP-1 released into the medium was inhibited by both IGF-I and follicle-stimulating hormone dose-dependently with an IC<sub>5o</sub> of 0.14 and 0.13 n M, respectively, while human chorionic gonadotropin-stimulated IGFBP-1 release with a 50% effective dose (ED<sub>50</sub>) of 0.6 n M. These results suggest that IGFBP-1 is involved in follicular development and granulosa cell differentiation in the human ovary and that gonadotropins may influence IGF-I action by modifying IGFBP-1 levels within the ovary.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          978-3-8055-5994-2
          978-3-318-01932-2
          1663-2818
          1663-2826
          1994
          1994
          05 December 2008
          : 41
          : Suppl 1
          : 22-28
          Affiliations
          aMaternal and Perinatal Center; bDepartment of Obstetrics and Gynecology, Tokyo Women’s Medical College, and cDepartment of Obstetrics and Gynecology, Ogikubo Hospital, Tokyo, Japan
          Article
          183939 Horm Res 1994;41:22–28
          10.1159/000183939
          7522203
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Local Regulators in the Ovary – Paracrine and Autocrine Control

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