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      A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages.

      The Journal of Immunology Author Choice
      Animals, CCAAT-Enhancer-Binding Proteins, Chromosome Mapping, Cloning, Molecular, DNA-Binding Proteins, deficiency, genetics, metabolism, physiology, Female, Gene Expression Regulation, immunology, Lectins, biosynthesis, Lectins, C-Type, Lipopolysaccharides, pharmacology, Macrophages, Peritoneal, Male, Membrane Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Transcription Factors, Transcription, Genetic

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          Abstract

          C-type lectins serve multiple functions through recognizing carbohydrate chains. Here we report a novel C-type lectin, macrophage-inducible C-type lectin (Mincle), as a downstream target of NF-IL6 in macrophages. NF-IL6 belongs to the CCAAT/enhancer binding protein (C/EBP) of transcription factors and plays a crucial role in activated macrophages. However, what particular genes are regulated by NF-IL6 has been poorly defined in macrophages. Identification of downstream targets is required to elucidate the function of NF-IL6 in more detail. To identify downstream genes of NF-IL6, we screened a subtraction library constructed from wild-type and NF-IL6-deficient peritoneal macrophages and isolated Mincle that exhibits the highest homology to the members of group II C-type lectins. Mincle mRNA expression was strongly induced in response to several inflammatory stimuli, such as LPS, TNF-alpha, IL-6, and IFN-gamma in wild-type macrophages. In contrast, NF-IL6-deficient macrophages displayed a much lower level of Mincle mRNA induction following treatment with these inflammatory reagents. The mouse Mincle proximal promoter region contains an indispensable NF-IL6 binding element, demonstrating that Mincle is a direct target of NF-IL6. The Mincle gene locus was mapped at 0.6 centiMorgans proximal to CD4 on mouse chromosome 6.

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