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      Role of Rev-erbα domains for transactivation of the connexin43 promoter with Sp1.

      Febs Letters

      Transcriptional Activation, Animals, metabolism, genetics, Sp1 Transcription Factor, chemistry, Repressor Proteins, Recombinant Proteins, Receptors, Cytoplasmic and Nuclear, Protein Interaction Domains and Motifs, Promoter Regions, Genetic, Nuclear Receptor Subfamily 1, Group D, Member 1, Mutant Proteins, Mutagenesis, Site-Directed, Mutagenesis, Insertional, Mice, Ligands, Humans, Heme, HEK293 Cells, Genes, Reporter, Gene Deletion, Connexin 43, Binding, Competitive

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          Abstract

          Rev-erbα, a component of the circadian clock, has also been known as a nuclear receptor that lacks activation function domain 2, functioning as a ligand-dependent transcriptional repressor. However, we recently reported that Rev-erbα activates connexin43 transcription by forming a complex with Sp1. Here we show that heme, a REV-ERB ligand, is dispensable for this novel mechanism and that Rev-erbβ, having homologies with Rev-erbα, does not activate connexin43, but competes with the Rev-erbα/Sp1. The A/B region of Rev-erbα, which is not conserved in Rev-erbβ, is a crucial activating domain, while the ligand binding domain, conserved in Rev-erbβ, functions as a competitor. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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          Author and article information

          Journal
          10.1016/j.febslet.2012.11.021
          23201262

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