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Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

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      The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay.

      A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, alpha-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 micromol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 micromol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.
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        Antioxidant activity applying an improved ABTS radical cation decolorization assay

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          Use of a free radical method to evaluate antioxidant activity

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            Author and article information

            Journal
            MOLEFW
            Molecules
            Molecules
            MDPI AG
            1420-3049
            July 2007
            July 19 2007
            : 12
            : 7
            : 1496-1547
            10.3390/12071496
            © 2007

            https://creativecommons.org/licenses/by/4.0/

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            Self URI (article page): http://www.mdpi.com/1420-3049/12/7/1496

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