In order to compare the effects of addition of fluorine (F) in diets differing in protein content on the urinary F excretion, blood profile and thyroid hormones, 30 crossbred calves (6-8 months) initially exposed to different protein levels were allotted into six groups in a 3 × 2 factorial design. The factors included three different levels of protein: normal (NP; 100%), low (LP; 75%), and high (HP; 125%) besides two levels of supplemental fluorine (as sodium fluoride) at 0 or 200 mg/kg diet. The animals were fed a wheat straw-based diet for 210 d. Feeding NP diets decreased urinary fluoride excretion, measured at 42 d intervals, compared with LP diets. Blood levels of hemoglobin and haematocrit, measured at 70 d intervals, were not affected by either protein or fluorine levels, but period differences were apparent. Serum levels of urea, alkaline phosphatase and F showed greater increase in groups supplemented with F than in those without it; however, serum calcium was higher in the latter. Serum tri-iodo-thyronine and thyroxine levels were higher in HP and NP fed calves than animals on LP diets, respectively. The animals fed LPF have higher urinary F as compared with animals fed NPF, but were not different from the group fed HPF. The blood and serum variables indicated that there is no extra protection against susceptibility to F toxicity upon feeding of 25 percent higher protein than requirements.