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      The potential and challenges of nanopore sequencing.

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          Abstract

          A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore. The nanopore provides a highly confined space within which single nucleic acid polymers can be analyzed at high throughput by one of a variety of means, and the perfect processivity that can be enforced in a narrow pore ensures that the native order of the nucleobases in a polynucleotide is reflected in the sequence of signals that is detected. Kilobase length polymers (single-stranded genomic DNA or RNA) or small molecules (e.g., nucleosides) can be identified and characterized without amplification or labeling, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility. Further research and development to overcome current challenges to nanopore identification of each successive nucleotide in a DNA strand offers the prospect of 'third generation' instruments that will sequence a diploid mammalian genome for approximately $1,000 in approximately 24 h.

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          Author and article information

          Journal
          Nat Biotechnol
          Nature biotechnology
          Springer Science and Business Media LLC
          1546-1696
          1087-0156
          Oct 2008
          : 26
          : 10
          Affiliations
          [1 ] Department of Molecular and Cell Biology, Harvard University, Cambridge, Massachusetts 02138, USA. dbranton@harvard.edu
          Article
          nbt.1495 NIHMS89972
          10.1038/nbt.1495
          2683588
          18846088
          b5f34604-7491-46b9-ad3d-d85552ad410e

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