To elucidate whether glucose absorption is necessary for oral glucose-induced truncated glucagon-like peptide-1 (tGLP-1) release, we examined tGLP-1 release by several stimulants from isolated perfused canine ileum. Glucose and nonabsorbable sugars, sucrose and 2-deoxyglucose (2DG), were intraluminally administrated. Following gel filtration of the perfusate after glucose ingestion, the main component of GLP-1 in the eluate is tGLP-1. Both glucose and tGLP-1 in the effluents increased after the intraluminal administration of 5% glucose, but not by that of sucrose or 2DG. Glucose absorption is more important than contact of glucose with ileal epithelium to stimulate tGLP-1 release.