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      The panE gene, encoding ketopantoate reductase, maps at 10 minutes and is allelic to apbA in Salmonella typhimurium.

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      Journal of bacteriology

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          Abstract

          In Salmonella typhimurium, precursors to the pyrimidine moiety of thiamine are synthesized de novo by the purine biosynthetic pathway or the alternative pyrimidine biosynthetic (APB) pathway. The apbA gene was the first locus defined as required for function of the APB pathway (D. M. Downs and L. Petersen, J. Bacteriol. 176:4858-4864, 1994). Recent work showed the ApbA protein catalyzes the NADPH-specific reduction of ketopantoic acid to pantoic acid. This activity had previously been associated with the pantothenate biosynthetic gene panE. Although previous reports placed panE at 87 min on the Escherichia coli chromosome, we show herein that apbA and panE are allelic and map to 10 min on both the S. typhimurium and E. coli chromosomes. Results presented here suggest that the role of ApbA in thiamine synthesis is indirect since in vivo labeling studies showed that pantoic acid, the product of the ApbA-catalyzed reaction, is not a direct precursor to thiamine via the APB pathway.

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          Author and article information

          Journal
          J. Bacteriol.
          Journal of bacteriology
          0021-9193
          0021-9193
          Sep 1998
          : 180
          : 17
          Affiliations
          [1 ] Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
          Article
          107496
          9721324
          b62c0260-abee-4299-aee1-e91f4eb7e6be
          History

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