5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Measuring the pH environment of DNA delivered using nonviral vectors: implications for lysosomal trafficking.

      1 ,
      Biotechnology and bioengineering
      Wiley

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The degradation of DNA in lysosomes represents a major obstacle to efficient nonviral gene delivery. The rational design of vectors that overcome this obstacle requires a better understanding of the lysosomal barrier to gene delivery, which in turn requires a means to investigate this intermediate step. To this end, we developed a technique to measure the pH environment of delivered DNA, from which the degree to which vectors avoided trafficking to acidic Iysosomes could be determined. The measured average pH of DNA delivered using poly-L-lysine (PLL) polyplexes was 4.5, suggesting that PLL polyplexes were trafficked to acidic lysosomes. Other vectors could avoid or buffer the pH of Iysosomes as DNA delivered using Lipofectamine Plus, polyethylenimine (PEI), linear polyethylenimine (LPEI), and two degradable poly(beta-amino ester)s (poly-1 and poly-2) had average pH values of 7.1, 5.9, 5.0, 6.7, and 6.4, respectively.

          Related collections

          Author and article information

          Journal
          Biotechnol. Bioeng.
          Biotechnology and bioengineering
          Wiley
          0006-3592
          0006-3592
          Jun 05 2002
          : 78
          : 5
          Affiliations
          [1 ] Department of Chemical Engineering, E25-342, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
          Article
          10.1002/bit.20215
          12115119
          b6de9366-ceee-4840-a15b-241bc99a2358
          History

          Comments

          Comment on this article