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      Effects of ethanol extract of Bombax ceiba leaves and its main constituent mangiferin on diabetic nephropathy in mice

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          Abstract

          The present study was designed to explore the mechanism by which ethanol extract of Bombax ceiba leaves (BCE) and its main constituent mangiferin (MGF) affect diabetic nephropathy by combating oxidative stress. Oral administration of BCE and MGF to normal and streptozotocin (STZ)-induced diabetic mice were carried out. Fasting blood glucose, 24-h urinary albumin, serum creatinine, and blood urea nitrogen were tested, histopathology, and immunohistochemical analysis of kidney tissues were performed. Moreover, mesangial cells were treated with BCE and MGF for 48 h with or without 25 mmol·L −1 of glucose. Immunofluorescence, Western blot and apoptosis analyses were used to investigate their regulation of oxidative stress and mitochondrial function. BCE and MGF ameliorated biochemical parameters and restored STZ-induced renal injury in the model mice. In vitro study showed that high glucose stimulation increased oxidative stress and cell apoptosis in mesangial cells. BCE and MGF limited mitochondrial membrane potential (ΔΨm) collapse by inhibiting Nox4, mitochondrially bound hexokinase II dissociation, and subsequent ROS production, which effectively reduced oxidative stress, cleaved caspase-3 expression and cell apoptosis. Our work indicated that BCE and MGF had protective effects on diabetic caused kidney injury and prevented oxidative stress in mesangial cells by regulation of hexokinase II binding and Nox4 oxidase signaling.

          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 August 2017
          : 15
          : 8
          : 597-605
          Affiliations
          [1] 1Department of Resources Science of Traditional Chinese Medicines, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, China
          [2] 2Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 211198, China
          Author notes
          *Corresponding author: Qin Min-Jian, Tel: 86-25-86185130, Fax: 86-25-85301528, E-mail: minjianqin@ 123456163.com

          ΔCo-first author.

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(17)30087-0
          10.1016/S1875-5364(17)30087-0
          28939022
          b78d442b-4add-4182-b2c7-5b7aafffc69e
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          History
          : 17 September 2016
          Funding
          Funded by: Preponderant Discipline Construction Project for Traditional Chinese Medicines of Jiangsu Province
          This work was supported by the Preponderant Discipline Construction Project for Traditional Chinese Medicines of Jiangsu Province.

          Medicine,Pharmaceutical chemistry,Pharmacology & Pharmaceutical medicine,Complementary & Alternative medicine
          Diabetic nephropathy,Mangiferin,Oxidative stress,Mitochondrial function, Bombax ceiba

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