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      Systematic review for geo-authentic Lonicerae Japonicae Flos

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          Abstract

          In traditional Chinese medicine, Lonicerae Japonicae Flos is commonly used as anti-inflammatory, antiviral, and antipyretic herbal medicine, and geo-authentic herbs are believed to present the highest quality among all samples from different regions. To discuss the current situation and trend of geo-authentic Lonicerae Japonicae Flos, we searched Chinese Biomedicine Literature Database, Chinese Journal Full-text Database, Chinese Scientific Journal Full-text Database, Cochrane Central Register of Controlled Trials, Wanfang, and PubMed. We investigated all studies up to November 2015 pertaining to quality assessment, discrimination, pharmacological effects, planting or processing, or ecological system of geo-authentic Lonicerae Japonicae Flos. Sixty-five studies mainly discussing about chemical fingerprint, component analysis, planting and processing, discrimination between varieties, ecological system, pharmacological effects, and safety were systematically reviewed. By analyzing these studies, we found that the key points of geo-authentic Lonicerae Japonicae Flos research were quality and application. Further studies should focus on improving the quality by selecting the more superior of all varieties and evaluating clinical effectiveness.

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          Lonicera japonica Thunb.: Ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine

          Graphical abstract Lonicera japonica has been used for thousands of years in China. Now, more than 140 chemical compounds have been isolated; and studies show Lonicera japonica possesses wide pharmacological actions. Meanwhile, it could be used as healthy food, ornamental groundcover, etc.
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            Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins.

            Chlorogenic acid, one of the most bioactive compounds rich in the Chinese medicinal herb honeysuckle, is a natural antioxidant and serves as anti-inflammatory, anti-tumor, anti-mutagenic and anti-carcinogenic agent. An efficient preparative separation process of chlorogenic acid from honeysuckle crude extracts has been developed in the present study. HPD-850 resin offers the best adsorption capacity, and adsorption and desorption ratios for chlorogenic acid among the nine macroporous resins tested, and its adsorption rate at 25 degrees C fit best to the Langmuir isotherm. The adsorption capacity of HPD-850 resin was found to depend strongly on the pH value of the initial adsorption solution. The dynamic adsorption and desorption experiments have been carried out on a HPD-850 resin packed column to optimize the separation process of chlorogenic acid from honeysuckle crude extracts. After one run treatment with HPD-850 resin, the chlorogenic acid content in the final product was increased 4.46-fold from 11.2% to 50.0%, with a recovery yield of 87.9%. The preparative separation of chlorogenic acid can be easily and efficiently achieved via adsorption and desorption on HPD-850 resin, and the method developed will provide a potential approach for large-scale separation and purification of chlorogenic acid for its wide pharmaceutical use.
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              Rapid quantitative analysis of adulterant Lonicera species in preparations of Lonicerae Japonicae Flos.

              Lonicerae Japonicae Flos is often adulterated with Lonicerae Flos, which is derived from the other four Lonicera species, in both the crude drug and Lonicerae Japonicae Flos preparations. We proposed a methodology for the quantitative analysis of adulterant Lonicerae Flos in Lonicerae Japonicae Flos preparations. Taking macranthoidins A, B, dipsacoside B (saponins), sweroside (iridoids), and luteolin-7-O-d-glucoside (flavonoids) as markers, a method of ultra high performance liquid chromatography with triple quadrupole mass spectrometry was employed to determine their amounts in Lonicerae Flos, Lonicerae Japonicae Flos, and Lonicerae Japonicae Flos preparations. The proportion of adulterant Lonicerae Flos in Lonicerae Japonicae Flos preparations was estimated based on the saponin contents of Lonicerae Japonicae Flos and Lonicerae Flos. All analytes separated under isocratic elution in 12 min with acceptable linearity, precision, repeatability, and accuracy. Lonicerae Japonicae Flos was easily distinguished from Lonicerae Flos by the total amount of saponins (0.067 and > 45.8 mg/g for Lonicerae Japonicae Flos and Lonicerae Flos, respectively). Eighteen of twenty one Lonicerae Japonicae Flos preparation samples were adulterated with Lonicerae Flos in proportions of 11.3-100%. The developed ultra high performance liquid chromatography with triple quadrupole mass spectrometry method could be used for the identification of Lonicerae Japonicae Flos and the four species of Lonicerae Flos and for the analysis of Lonicerae Japonicae Flos preparations adulterated with Lonicerae Flos.
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                Author and article information

                Contributors
                hely3699@163.com
                Journal
                Front Med
                Front Med
                Frontiers of Medicine
                Higher Education Press (Beijing )
                2095-0217
                2095-0225
                19 April 2017
                2017
                : 11
                : 2
                : 203-213
                Affiliations
                [1 ]ISNI 0000 0001 1431 9176, GRID grid.24695.3c, Beijing University of Chinese Medicine, ; Beijing, 100029 China
                [2 ]ISNI 0000 0004 0632 3409, GRID grid.410318.f, State Key Laboratory Breeding Base of Dao-di Herbs, , China Academy of Chinese Medical Sciences, ; Beijing, 100700 China
                [3 ]ISNI 0000 0004 0632 3409, GRID grid.410318.f, Institute of Basic Research in Clinical Medicine, , China Academy of Chinese Medical Sciences, ; Beijing, 100700 China
                [4 ]ISNI 0000 0004 0632 3409, GRID grid.410318.f, Xiyuan Hospital, , China Academy of Chinese Medical Sciences, ; Beijing, 100091 China
                [5 ]GRID grid.412643.6, The First Clinical Medical College of Lanzhou University, ; Lanzhou, 730000 China
                Article
                504
                10.1007/s11684-017-0504-0
                7089257
                28425044
                b7a59cd2-2938-4c24-ab4b-08bdf3907fac
                © Higher Education Press and Springer-Verlag Berlin Heidelberg 2017

                This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

                History
                : 25 April 2016
                : 27 November 2016
                Categories
                Review
                Custom metadata
                © Higher Education Press and Springer-Verlag GmbH Germany 2017

                lonicerae japonicae flos,geo-authentic,herbal quality,chemical fingerprint,component analysis

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