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      The Metabolism of Methylene Blue and Its Derivatives in Japanese Eel ( Anguilla Japonica)

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          Methylene blue (MB) is commonly used in aquaculture as a fungicide and antidotes. This study was designed to explore the pharmacokinetics of MB in Japanese eel ( Anguilla Japonica) immersed in 10 mg L −1 and 20 mg L −1 MB for 0.5 h. The concentrations of MB and its derivatives in the blood, liver, kidney, skin and muscle were determined by HPLC after immersion. The results showed that the changes of drug concentrations in Japanese eel were basically the same in different dose groups, showing a general trend of increasing at first and then decreasing, but the peak time was slightly different. The peak concentration of the drug was positively correlated with the dose level. The peak concentrations of MB in MB (20 mg L −1) group, MB, azure A and azure B in the tissues of Japanese eel were significantly higher than those in MB (10 mg L −1) group. Moreover, MB, azure A and azure B remained for a long time and could still be detected at 64 days, and azure C was not detected in tissues.

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          Author and article information

          Journal of Ocean University of China
          Science Press and Springer (China )
          02 May 2020
          01 June 2020
          : 19
          : 3
          : 653-658
          1National Pathogen Collection Center for Aquatic Animals, Shanghai 201306, China
          2National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
          3Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China
          4East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
          Author notes
          *Corresponding author: HU Kun, E-mail: khu@

          # The two authors contributed equally to this work.

          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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