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      Holographic coherent anti-Stokes Raman scattering bio-imaging

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          Abstract

          CARS holography captures both the amplitude and the phase of a complex anti-Stokes field, and can perform three-dimensional imaging by digitally focusing onto different depths inside a specimen. The application of CARS holography for bio-imaging is demonstrated. It is shown that holographic CARS imaging of sub-cellular components in live HeLa cells can be achieved.

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          Most cited references11

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          Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering

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            Coherent anti‐Stokes Raman spectroscopy

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              Compressive holography.

              Compressive sampling enables signal reconstruction using less than one measurement per reconstructed signal value. Compressive measurement is particularly useful in generating multidimensional images from lower dimensional data. We demonstrate single frame 3D tomography from 2D holographic data.
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                Author and article information

                Journal
                Biomed Opt Express
                Biomed Opt Express
                BOE
                Biomedical Optics Express
                Optical Society of America
                2156-7085
                27 June 2012
                01 July 2012
                27 June 2012
                : 3
                : 7
                : 1744-1749
                Affiliations
                [1 ]Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802, USA
                [2 ]State Key Laboratory for Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, China
                [3 ]Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA
                [4 ]School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
                Author notes
                Article
                168344
                10.1364/BOE.3.001744
                3395496
                22808443
                b7c432b0-9e36-49c5-ab13-daedf0fc0274
                ©2012 Optical Society of America

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

                History
                : 15 May 2012
                : 25 June 2012
                : 25 June 2012
                Funding
                Funded by: National Science Foundation of China
                Award ID: NSFC# 11174019
                Award ID: 11121091
                Funded by: National Institute of Health
                Award ID: R01CA136856
                Funded by: Nano-Tera
                Award ID: #530072 “NT-SSSTC-Optofluid”
                Funded by: National Science Foundation
                Award ID: ECCS-0547475
                Award ID: DBI-0649866
                Categories
                Microscopy
                Custom metadata
                True
                0

                Vision sciences
                (090.1995) digital holography,(180.6900) three-dimensional microscopy,(180.5655) raman microscopy

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