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      Occurrence of bovine dermatophilosis in Huambo province, Angola Translated title: Ocurrencia de dermatofilosis bovina en la provincia Huambo, Angola

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          Abstract

          Dermatofilosis es una dermatitis pustular exudativa que afecta al ganado bovino, ovejas, caballos, cabras, perros, gatos, reptiles y, ocasionalmente, a humanos. Esta enfermedad es causada por Dermatophilus congolensis, un actinomiceto pleomórfico Gram-positivo. Según los Servicios Veterinarios, la mayor incidencia de la enfermedad en Angola se identificó en las provincias Huambo, Huila, Kwanza Sul y Malang durante mayo de 2013 y abril de 2015, pero solo se basó en lesiones clínicas. En el presente trabajo se obtuvieron 90 muestras de costra de animales con lesiones en la piel en Huambo, Angola, en mayo de 2013 y abril de 2015. Las bacterias se aislaron siguiendo el método de Haalstra. A las colonias presuntivas del género Dermathophilus se les realizó tinción de Gram, identificación bioquímica y detección molecular por PCR. Se seleccionaron ocho aislamientos de Dermathophilus congolensis para su sensibilidad a 11 antibióticos diferentes. Un total de 30 muestras mostraron colonias típicas del género Dermatophilus. El amplicón de 817 pb correspondiente a la región ARNr 16S se obtuvo en treinta cepas sospechosas de D. congolensis. La prueba de sensibilidad a las drogas reveló que Amoxicilina/clavulanato, Clindamicina, Eritromicina, Lincomicina, Azitromicina, Rifampicina y Enrofloxacina fueron altamente eficaces contra los ocho aislados de D. congolensis. La observación de lesiones y la identificación por técnicas bioquímicas y moleculares confirmaron la dermatophilosis bovina en la región de Huambo, Angola

          Translated abstract

          Dermatophilosis is an exudative pustular dermatitis that affects cattle, sheep, horses, goats, dogs, cats, reptiles, and occasionally humans. This disease is caused by Dermatophilus congolensis, a pleomorphic Gram-positive actinomycete. According to the Veterinary Services, the highest incidence of the disease in Angola was identified in the provinces Huambo, Kwanza Sul and Malang during May 2013 and April 2015, but only supported on clinical sings. In the present work ninety crust samples were taken from animals with lesions on the skin in Huambo province, in the period May 2013-April 2015. The bacteria were isolated following Haalstra´s method. Presumptive colonies of the Dermathophilus genus were subjected to Gram stain, biochemical identification, and molecular detection by PCR. Eight Dermathophilus congolensis isolates were tested for their sensitivity to eleven different antibiotics. A total of 30 samples showed typical colonies of the Dermatophilus genus. The amplicon of 817 bp corresponding to the 16S rRNA region was obtained in the 30 isolates suspecting of being D. congolensis. Drug sensitivity test revealed that Amoxicillin/clavulanate, Clindamycin, Erythromycin, Lincomycin, Azithromycin, Rifampicin, and Enrofloxacin were highly effective against the eight D. congolensis isolates. Observation of lesions and microbiologic diagnosis using biochemical and molecular techniques allowed confirming bovine dermatophilosis in the region of Huambo, Angola

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          Most cited references21

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          Fórmulas para el cálculo de la muestra en investigaciones de salud

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            Is infection by Dermatophilus congolensis underdiagnosed?

            Dermatophilus congolensis, which affects animal species, is an uncommon human infection. Few cases, mainly in tropical areas, have been reported. We describe the first human infection in Spain in a traveler returning from Central America. Diagnosis of human infection may be underestimated in people in contact with animals.
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              Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

              Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rsa
                Revista de Salud Animal
                Rev Salud Anim.
                Centro Nacional de Sanidad Agropecuaria (La Habana, , Cuba )
                0253-570X
                2224-4700
                August 2017
                Affiliations
                [01] orgnameJose Eduardo Dos Santos University orgdiv1Faculty of Veterinary Medicine Angola
                [02] San José de las Lajas Mayabeque orgnameNational Center for Animal and Plant Health orgdiv1Departament of Microbiology-Epidemiology Cuba
                Article
                S0253-570X2017000200004
                b7f30a9f-86a5-4d53-83f7-fb8c2316b343

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 06 February 2017
                : 09 June 2017
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 30, Pages: 0
                Product

                SciELO Cuba


                antimicrobial resistance,diagnosis,bovine,Dermatophilus congolensis,diagnóstico,resistencia antimicrobiana,bovino,Dermathophilus congolensis

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