Flow cytometric kinetic assay of the activity of Na+/H+ antiporter in mammalian cells.

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Abstract

The Na(+)/H(+) exchanger (NHE) of mammalian cells is an integral membrane protein that extrudes H(+) ion in exchange for extracellular Na(+) and plays a crucial role in the regulation of intracellular pH (pHi). Thus, when pHi is lowered, NHE extrudes protons at a rate depending of pHi that can be expressed as pH units/s.

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Journal

Journal ID (iso-abbrev): Cytometry A

Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology

Publisher: Wiley

ISSN: 1552-4922

ISSN (Print): 1552-4922

Publication date (Electronic): Oct 2004

Volume: 61

Issue: 2

Affiliations

[1 ] Unidad de Investigaciones Cardíacas, Departamento de Biología y Terapia Molecular y Celular, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas, Valencia, Spain.

Article

DOI: 10.1002/cyto.a.20077

PubMed ID: 15382148

SO-VID: b7f8f890-0c36-4669-924d-f3f6b8e9fcc2

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