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      Immobilization of Microbes for Bioremediation of Crude Oil Polluted Environments: A Mini Review

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          Abstract

          Petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. Oil pollution may arise either accidentally or operationally whenever oil is produced, transported, stored and processed or used at sea or on land. Oil spills are a major menace to the environment as they severely damage the surrounding ecosystems. To improve the survival and retention of the bioremediation agents in the contaminated sites, bacterial cells must be immobilized. Immobilized cells are widely tested for a variety of applications. There are many types of support and immobilization techniques that can be selected based on the sort of application. In this review article, we have discussed the potential of immobilized microbial cells to degrade petroleum hydrocarbons. In some studies, enhanced degradation with immobilized cells as compared to free living bacterial cells for the treatment of oil contaminated areas have been shown. It was demonstrated that immobilized cell to be effective and is better, faster, and can be occurred for a longer period

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          Most cited references80

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          Organic polymer supports for synthesis and for reagent and catalyst immobilization.

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            Microencapsulation of microbial cells.

            The high level of biocatalysts such as microbial cells and enzymes plays an important role in increasing the productivity of a bioreactor. The beads entrapped with microbial cells are not strong enough for long-term use. The small void space of polymer matrix and the leakage of cells limit a final cell loading in the beads. The recent success of encapsulating microbial cells makes it possible to prepare dense biocatalyst composed of recombinant microbial cells. In addition to encapsulating microbial cells, immobilization of animal and plant cells in capsules is also briefly described.
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              Immobilisation of enzymes on mesoporous silicate materials.

              Mesoporous silicates (MPS) are attractive materials for the immobilisation of enzymes. They possess ordered pore structures, narrow pore size distributions, large surface areas, high stability and can be chemically modified with various functional groups. The properties of MPS materials are reviewed in terms of their ability to act as supports for enzymes for use in biocatalysis with a particular focus on the ability to tailor the surface functionalization of the MPS to suit a specific enzyme. While many reports of the immobilisation of enzymes on MPS have been described, their use as biocatalytic supports is limited. Large scale reactors based on MPS will require continuous flow systems where the properties of the support can be tailored while allowing fluid flow at reasonable low pressure.
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                Author and article information

                Journal
                Open Microbiol J
                Open Microbiol J
                TOMICROJ
                The Open Microbiology Journal
                Bentham Open
                1874-2858
                31 July 2015
                2015
                : 9
                : 48-54
                Affiliations
                [1 ]Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
                [2 ]Institute for Marine Environment Research (IAMC), CNR of Messine, Messina, Italy
                Author notes
                [* ] Address correspondence to this author at the Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran; Tel: +989132906971; Fax: +9834322032; E-mail: mshahi@ 123456uk.ac.ir
                Article
                TOMICROJ-9-48
                10.2174/1874285801509010048
                4676050
                26668662
                b80f2acb-c3d6-49e4-a6ca-92276d096d72
                © Bayat et al.; Licensee Bentham Open.

                This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

                History
                : 01 December 2014
                : 13 April 2015
                : 14 April 2015
                Categories
                Article

                Microbiology & Virology
                application, bacteria,biodegradation,crude oil,soil.
                Microbiology & Virology
                application, bacteria, biodegradation, crude oil, soil.

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