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      Changes of the cAMP-Dependent Protein Kinases during Differentiation of Lens Cells

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          Several changes occur in the properties of cAMP-dependent protein kinase during the differentiation of lens epithelial cells, and/or in the course of aging of fiber cells. Two types of isoenzymes (I and II) were found on DEAE-cellulose chromatography of epithelial cells. The chromatography of the crude extract of cortical fibers yielded one protein kinase fraction only, in the position of type I kinase. A sharp cAMP-dependent protein kinase peak was observed in the position of isoenzyme II on DEAE, and several lower molecular weight, cAMP-dependent protein kinase fractions appeared in Sephadex G-200 after mild acid treatment (pH 4.8) of the cortical crude extract. <sup>3</sup>H-cAMP saturation curve (Scatchard plot) of acid-treated protein kinase showed the loss of positive cooperativity. Aggregation of the I and II isoenzymes during lens cell differentiation is suggested.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          03 December 2009
          : 11
          : 5-6
          : 366-371
          1st Institute of Biochemistry, Semmelweis University Medical School, Budapest
          265035 Ophthalmic Res 1979;11:366–371
          © 1979 S. Karger AG, Basel

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          Page count
          Pages: 6
          Growth Control, Differentiation, and Ageing of the Eye Lens


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