39
views
0
recommends
+1 Recommend
0 collections
    4
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The present paper describes important features of the immune response induced by the Cry1Ac protein from Bacillus thuringiensis in mice. The kinetics of induction of serum and mucosal antibodies showed an immediate production of anti-Cry1Ac IgM and IgG antibodies in serum after the first immunization with the protoxin by either the intraperitoneal or intragastric route. The antibody fraction in serum and intestinal fluids consisted mainly of IgG1. In addition, plasma cells producing anti-Cry1Ac IgG antibodies in Peyer's patches were observed using the solid-phase enzyme-linked immunospot (ELISPOT). Cry1Ac toxin administration induced a strong immune response in serum but in the small intestinal fluids only anti-Cry1Ac IgA antibodies were detected. The data obtained in the present study confirm that the Cry1Ac protoxin is a potent immunogen able to induce a specific immune response in the mucosal tissue, which has not been observed in response to most other proteins.

          Related collections

          Author and article information

          Journal
          Braz J Med Biol Res
          Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
          FapUNIFESP (SciELO)
          0100-879X
          0100-879X
          Feb 2000
          : 33
          : 2
          Affiliations
          [1 ] Center for Genetic Engineering and Biotechnology, Havana, Cuba. roberto.vasquez@cigb.edu.cu
          Article
          S0100-879X2000000200002
          10.1590/s0100-879x2000000200002
          10657055
          b921f966-dace-41f6-ad54-dbad2e3d0b20
          History

          Comments

          Comment on this article