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      Effects of Different Preservation Methods on Physicochemical Property of Marine Pathogen Vibrio anguillarum

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          Freeze-drying, continuous passage and ultra-low temperature cryopreservation are often used to preserve pathogens. In this study, Vibrio anguillarum was rejuvenated by intramuscular infection as the initial strain. The difference between cells preserved with different preservation methods and their initial strains were compared with physiological and biochemical methods and through antibiotics resistance analysis. The composition of protectants for freeze-drying V. anguillarum was optimized. We found that the optimal composition of protectants was 8% of trehalose, 12% of skim milk, 8.0% of lactose, 2.0% of sodium citrate, 12.0% of serum and 8.0% of mannitol. The indexes of lysine decarboxylase and urease changed after continuous passage. The urease reaction changed after freeze-drying and freeze-thawing, but the reaction can be restored to the initial after freeze-drying. Based on the antibiotics resistance analyses, the sensitivity of V. anguillarum to different drugs including rifampicin, erythrocin, furazolidone, ceftazidime, lomefloxacin, gentamycin, azithromycin, doxycycline, ampicillin, co-trimoxazole and cefoperazone changed after different treatments, and some of these changes can be restored to the original through activation culture. In sum, compared with cryopreservation and continuous passage, the freeze-drying is more sustainable for the long-term preservation of V. anguillarum, which showed a better effect in maintaining the original characteristics of pathogen.

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          Author and article information

          Journal of Ocean University of China
          Science Press and Springer (China )
          12 November 2019
          01 December 2019
          : 18
          : 6
          : 1417-1426
          1Laboratory for Marine Fisheries Science and Food Production Processes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
          Author notes
          *Corresponding authors: ZHANG Zheng and WANG Yingeng
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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