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      Electrochemical Sunset Yellow Biosensor Based on Photocured Polyacrylamide Membrane for Food Dye Monitoring

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          Abstract

          An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide- co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide- co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0–101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection.

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          Most cited references46

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          Synthesis, characterization and rheological behavior of pH sensitive poly(acrylamide-co-acrylic acid) hydrogels

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            Simultaneous determination of Sunset yellow and Tartrazine in soft drinks using gold nanoparticles carbon paste electrode.

            The monitoring of synthetic dyes in foods is very important due to their potential harmfulness to human beings. Herein, a carbon-paste electrode (CPE) that is chemically modified with gold nanoparticles (nAu) was fabricated and used for the determination of Sunset yellow (SY) and Tartrazine (Tz). Cyclic and differential pulse voltammetry (CV and DPV) results revealed two well-resolved anodic peaks for SY and Tz with remarkably increase in oxidation signals of these colourants. Based on this, a novel electrochemical method was developed for the simultaneous determination of SY and Tz. High sensitivity and selectivity, sub-micromolar detection limit, high reproducibility and regeneration of the electrode surface by simple polishing make the nAu-CPE electrode very suitable for the determination of SY and Tz in commercially available soft drinks. The detection limits was 3.0×10(-8) and 2.0×10(-9)moll(-1) for SY and Tz, respectively, which are remarkably lower than those reported previously for SY and Tz using other modified electrodes.
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              Optimization of Parameters for Adsorption of Methylene Blue on a Low-Cost Activated Carbon

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                Author and article information

                Journal
                Sensors (Basel)
                Sensors (Basel)
                sensors
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                01 January 2018
                January 2018
                : 18
                : 1
                : 101
                Affiliations
                [1 ]School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia; adizam_92@ 123456yahoo.com (N.R.); maynotemus@ 123456yahoo.com (A.A.); leeyookheng@ 123456yahoo.co.uk (L.Y.H.)
                [2 ]Fuel Cell Institute, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia; ksloh@ 123456ukm.edu.my
                [3 ]Polymer Research Center, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
                Author notes
                [* ]Correspondence: sharina@ 123456ukm.edu.my ; Tel.: +603-8921-3902
                Article
                sensors-18-00101
                10.3390/s18010101
                5796456
                29301262
                b94fe38b-1f0f-422d-b0c5-840af8c9bbd7
                © 2018 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 04 November 2017
                : 15 December 2017
                Categories
                Article

                Biomedical engineering
                electrochemical biosensor,sunset yellow,synthetic food dye,laccase,photopolymerization

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