We evaluated the tRNA-derived fragments from human prostate tissue that were discovered in deep-sequencing (RNA-seq) data and reported in [1]. Our study of the same RNA-seq datasets reveals a considerably different and more populous pool of tRNA fragments, many of them with higher abundance than the fragments that were reported originally. We also evaluated the employed q-PCR technique. As the latter lacks 5'-endpoint specificity, it will not estimate correctly the abundance of many of the tRFs that are present in the sampled RNA populations from human prostate tissue.