For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
13
views
0
references
 Top references
cited by
0
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      75
      similar
       All similar
      Are you tired of sifting through news that doesn't interest you?
      Personalize your Karger newsletter today and get only the news that matters to you!

      Sign up

      • Record: found
      • Abstract: found
      • Article: found

      Identification and Partial Purification of a Nucleotide-Stimulated Protease in Rat Retina

      research-article

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A nucleotide-stimulated protease of rat retina was partially purified by DEAE-cellulose column chromatography. A 50-fold increase of an ATP-stimulated protease activity over a cytosol fraction was achieved by this chromatography. Maximum stimulation of protease activity by ATP was achieved at 2 mM. The pH optimum of the ATP-stimulated protease activity was between 7.5 and 8.5. 2 mM GTP stimulated the protease activity by 91%. 5 mM cyclic AMP stimulated the protease activity by 73% while 5 mM cyclic GMP stimulated the activity by 84%. The possible role of the nucleotide-stimulated protease in retina and potential involvement of the protease with inherited retinal dystrophy are discussed.

          Related collections

          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1985
          Publication date (Print): 1985
          Publication date (Electronic): 04 December 2009
          Volume: 17
          Issue: 5
          Pages: 282-288
          Affiliations
          Department of Ophthalmology and Visual Sciences, and Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Tex., USA
          Article
          Publisher ID: 265387 Other ID: Ophthalmic Res 1985;17:282–288
          DOI: 10.1159/000265387
          PubMed ID: 2999672
          SO-VID: b9883379-a436-40c1-b6bb-6cb128a365b7
          Copyright © © 1985 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 22 June 1984
          Date accepted : 13 December 1984
          Page count
          Pages: 7
          Categories
          Subject: Original Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Retinal degeneration,Protein degradation pathway,Cyclic nucleotide-stimulated protease,Nucleotide-stimulated protease,Retina
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Retinal degeneration, Protein degradation pathway, Cyclic nucleotide-stimulated protease, Nucleotide-stimulated protease, Retina

          Comments

          Comment on this article