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      Anticataract Action of Vitamin E: Its Estimation Using an in vitro Steroid Cataract Model


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          The aim of this study was to estimate the anticataract action of vitamin E using an in vitro methylprednisolone (MP)-induced cataract model. The same severity of early cortical cataract was induced in lenses isolated from male Wistar rats aged 6 weeks by incubation with MP (1.5 mg/ml) in TC-199 medium. The cataractous lenses showed slight increases in lipid peroxide (LPO) content and Na<sup>+</sup>/K<sup>+</sup> ratio and slight decreases in reduced glutathione (GSH) content and glyceraldehyde-3-phosphate dehydrogenase (GAP-DH), a sensitive index of oxidative stress, and Na<sup>+</sup>, K<sup>+</sup>-ATPase activities. When the cataractous lenses were further incubated in TC-199 medium with and without vitamin E (250 μg/ml) for 48 h, the progression of cataract was prevented in the vitamin E-treated lenses, but not in the vitamin E-untreated lenses. The vitamin E-untreated lenses showed a decrease in vitamin E content and an increase in water content in addition to further increases in LPO content and Na<sup>+</sup>/K<sup>+</sup> ratio and further decreases in GSH content and GAP-DH and Na<sup>+</sup>, K<sup>+</sup>-ATPase activities. In contrast, the changes of these components and enzymes except for GSH were attenuated in the vitamin E-treated lenses. From these results, it can be estimated that vitamin E prevents in vitro cataractogenesis in rat lenses treated with MP by protecting the lenses against oxidative damage and loss of membrane function.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 28
          : Suppl 2
          : 16-25
          Departments of aBiochemistry and bOphthalmology, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan
          267952 Ophthalmic Res 1996;28:16–25
          © 1996 S. Karger AG, Basel

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          Page count
          Pages: 10

          Vision sciences,Ophthalmology & Optometry,Pathology
          Methylprednisolone,Membrane dysfunction,Cultured rat lens,Vitamin E Oxidative damage,Steroid cataract


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