CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus.

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.

Related collections

Author and article information

Journal

Journal ID (iso-abbrev): J. Microbiol. Methods

Title: Journal of microbiological methods

Publisher: Elsevier BV

ISSN (Electronic): 1872-8359

ISSN (Print): 0167-7012

Publication date (Electronic): May 15 2017

Volume: 139

Affiliations

[1 ] Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, China.

[2 ] Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, China. Electronic address: jinyingli@jlu.edu.cn.

[3 ] Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China.

[4 ] Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address: wanglin1020@jlu.edu.cn.

[5 ] Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China. Electronic address: wangtd@jlu.edu.cn.

[6 ] Department of Biochemistry and Molecular Biology, College of Animal Science, Jilin University, Changchun, China. Electronic address: wangdc@jlu.edu.cn.

Article

Publisher Item ID: S0167-7012(17)30118-5

DOI: 10.1016/j.mimet.2017.05.008

PubMed ID: 28522389

SO-VID: b9e728aa-1dd8-43de-aefa-38aa7b0779ea

History

Keywords: CRISPR interference,Gene knockdown,Staphylococcus aureus

Data availability:

Keywords: CRISPR interference, Gene knockdown, Staphylococcus aureus

Comments

Comment on this article

scite_

Cited by 10

See all cited by